Suzuki S, Atai H, Hatakeyama Y, Hara M, Nakagawa S
Preclinical Research Laboratories, Central Institute for Experimental Animals, Kawasaki, Japan.
Mutat Res. 1989 Aug;223(4):407-10. doi: 10.1016/0165-1218(89)90097-9.
The effect of route of administration on the outcome of the micronucleus test was studied in 2 laboratories by administering the model chemical benzene intraperitoneally (i.p.) and orally (p.o.) to 2 strains of mice: MS/Ae and CD-1. On the basis of results obtained in a small-scale acute toxicity study and in a pilot micronucleus test, full-scale micronucleus tests were performed with a 24-h sampling time at doses of 250, 500, 1000, and 2000 mg/kg i.p. and 500, 1000, 2000, and 4000 mg/kg p.o. In both strains of mice, a higher incidence of micronucleated polychromatic erythrocytes (MNPCEs) was observed after p.o. administration. The ratio of polychromatic erythrocytes (PCEs) to total erythrocytes decreased more markedly at higher doses i.p. in both strains. Thus, benzene induced more micronuclei via the p.o. route, while inhibitory effects on bone marrow cells were stronger after i.p. administration.
两个实验室通过向MS/Ae和CD-1两种品系的小鼠腹腔内（i.p.）和口服（p.o.）给予模型化学物质苯，研究了给药途径对微核试验结果的影响。根据小规模急性毒性研究和预试验微核试验获得的结果，在给药后24小时取样时间下，以250、500、1000和2000 mg/kg腹腔注射及500、1000、2000和4000 mg/kg口服的剂量进行了全面的微核试验。在两种品系的小鼠中，口服给药后观察到微核多染性红细胞（MNPCEs）的发生率更高。在两种品系中，腹腔注射较高剂量时多染性红细胞（PCEs）与总红细胞的比例下降更为明显。因此，苯经口服途径诱导产生更多微核，而腹腔注射给药后对骨髓细胞的抑制作用更强。
