Feyder Michael, Goff Loyal A
J Clin Invest. 2016 Aug 1;126(8):2783-91. doi: 10.1172/JCI84422.
The number of long noncoding RNAs (lncRNAs) has grown rapidly; however, our understanding of their function remains limited. Although cultured cells have facilitated investigations of lncRNA function at the molecular level, the use of animal models provides a rich context in which to investigate the phenotypic impact of these molecules. Promising initial studies using animal models demonstrated that lncRNAs influence a diverse number of phenotypes, ranging from subtle dysmorphia to viability. Here, we highlight the diversity of animal models and their unique advantages, discuss the use of animal models to profile lncRNA expression, evaluate experimental strategies to manipulate lncRNA function in vivo, and review the phenotypes attributable to lncRNAs. Despite a limited number of studies leveraging animal models, lncRNAs are already recognized as a notable class of molecules with important implications for health and disease.
长链非编码RNA（lncRNA）的数量增长迅速；然而，我们对其功能的了解仍然有限。尽管培养细胞有助于在分子水平上研究lncRNA的功能，但使用动物模型能提供丰富的背景来研究这些分子的表型影响。使用动物模型进行的有前景的初步研究表明，lncRNA会影响多种表型，从细微的畸形到生存能力。在这里，我们强调动物模型的多样性及其独特优势，讨论使用动物模型来分析lncRNA表达，评估在体内操纵lncRNA功能的实验策略，并综述可归因于lncRNA的表型。尽管利用动物模型进行的研究数量有限，但lncRNA已被公认为是一类对健康和疾病具有重要影响的显著分子。
