Rodrigues Carmem Regine Faleiro, Plentz Luciana Ciarelli, Marcucci Maria Cristina, Dihl Rafael Rodrigues, Lehmann Mauricio
PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Universidade Luterana do Brasil, ULBRA, Canoas, Rio Grande do Sul, Brazil.
Universidade Bandeirante de São Paulo (UNIBAN), Pós-graduação em Farmácia, São Paulo, São Paulo, Brazil.
Food Chem Toxicol. 2016 Oct;96:117-21. doi: 10.1016/j.fct.2016.07.037. Epub 2016 Jul 30.
Propolis is a resinous, complex mixture of compounds collected by the bee species Apis mellifera. This study investigated the genotoxicity of green and brown propolis collected in southeast Brazil using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. The effect of five concentrations (0.5, 1.0, 2.0, 4.0, and 7.5 mg/mL) of both propolis types was analyzed in standard (ST) and high-bioactivation (HB) crosses, which have normal and high levels of cytochrome P450 enzymes, respectively. The results show that the types of propolis evaluated have no mutagenic action, in either cross. Moreover, chromatography findings revealed that the propolis types analyzed have different chemical compositions. Brown propolis had lower levels of polyphenols (∼7.2 mg/mL), compared to the green type (34.9 mg/g). Taken together, the findings of the present study and literature reports point to the safety in consuming low amounts of propolis, considering the risk of genetic damage, and confirm the absence of mutagenic and recombinagenic actions of the propolis types investigated.
蜂胶是蜜蜂采集的一种树脂状、复杂的化合物混合物。本研究使用果蝇的体细胞突变和重组试验（SMART），调查了在巴西东南部采集的绿色和棕色蜂胶的遗传毒性。在标准（ST）杂交和高生物活化（HB）杂交中分析了两种蜂胶类型的五种浓度（0.5、1.0、2.0、4.0和7.5毫克/毫升）的影响，这两种杂交分别具有正常水平和高水平的细胞色素P450酶。结果表明，所评估的蜂胶类型在两种杂交中均无诱变作用。此外，色谱分析结果显示，所分析的蜂胶类型具有不同的化学成分。与绿色蜂胶（34.9毫克/克）相比，棕色蜂胶的多酚含量较低（约7.2毫克/毫升）。综合来看，考虑到遗传损伤风险，本研究结果和文献报告表明食用少量蜂胶是安全的，并证实所研究的蜂胶类型不存在诱变和重组作用。
