Machado Nayane Moreira, de Rezende Alexandre Azenha Alves, Nepomuceno Júlio César, Tavares Denise Crispim, Cunha Wilson Roberto, Spanó Mário Antônio
Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Campus Umuarama, Uberlândia, Minas Gerais, Brazil.
Universidade Federal de Uberlândia, Faculdade de Ciências Integradas do Pontal, Ituiutaba, Minas Gerais, Brazil.
Food Chem Toxicol. 2016 Oct;96:226-33. doi: 10.1016/j.fct.2016.08.005. Epub 2016 Aug 4.
The main of this study was to evaluate the mutagenic and carcinogenic potential of (+) - usnic acid (UA), using Somatic Mutation and Recombination Test (SMART) and the test for detecting epithelial tumor clones (wts) in Drosophila melanogaster. Larvae from 72 ± 4 h from Drosophila were fed with UA (5.0, 10.0 or 20.0 mM); urethane (10.0 mM) (positive control); and solvent (Milli-Q water, 1% Tween-80 and 3% ethanol) (negative control). ST cross produced increase in total mutant spots in the individuals treated with 5.0, 10.0 or 20.0 mM of UA. HB cross produced spot frequencies in the concentration of 5.0 mM that were higher than the frequency for the same concentration in the ST cross. In the highest concentrations the result was negative, which means that the difference observed can be attributed, in part, to the high levels of P450, suggesting that increasing the metabolic capacity maximized the toxic effect of these doses. In the evaluation of carcinogenesis using the wts test, the results obtained for the same concentrations of UA show a positive result for the presence of tumors when compared to the negative control. We conclude that UA has recombinogenic, mutagenic and carcinogenic effects on somatic cells in D. melanogaster.
本研究的主要目的是利用体细胞突变和重组试验（SMART）以及检测黑腹果蝇上皮肿瘤克隆（wts）的试验，评估（+）-松萝酸（UA）的致突变和致癌潜力。用UA（5.0、10.0或20.0 mM）、氨基甲酸乙酯（10.0 mM）（阳性对照）和溶剂（超纯水、1%吐温-80和3%乙醇）（阴性对照）喂养72±4小时龄的黑腹果蝇幼虫。ST杂交在经5.0、10.0或20.0 mM UA处理的个体中产生的总突变斑点增加。HB杂交在5.0 mM浓度下产生的斑点频率高于ST杂交中相同浓度下的频率。在最高浓度下结果为阴性，这意味着观察到的差异部分可归因于高水平的P450，表明代谢能力的增加使这些剂量的毒性作用最大化。在使用wts试验评估致癌作用时，与阴性对照相比，相同浓度UA获得的结果显示出肿瘤存在的阳性结果。我们得出结论，UA对黑腹果蝇的体细胞具有重组、致突变和致癌作用。
