Machado N M, Lopes J C, Saturnino R S, Fagan E B, Nepomuceno J C
Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Bloco 2E, Campus Umuarama, Uberlândia, Minas Gerais, Brazil; Centro Universitário de Patos de Minas, Laboratório de Citogenética e Mutagênese, Patos de Minas, Minas Gerais, Brazil.
Food Chem Toxicol. 2013 Dec;62:355-60. doi: 10.1016/j.fct.2013.08.051. Epub 2013 Aug 28.
Carbon nanotubes (CNTs) are formed by rolling up a single graphite sheet into a tube. Among the different types of CNTs, the multi-walled carbon nanotubes (MWCNTs) comprise a set of concentric nanotubes with perfect structures. Several uses for MWCNTs have been suggested to be included in biological applications such as manufacturing of biosensors, carriers of drugs. However, before these materials can be put on the market, it is necessary to know their genotoxic effects. Thus, this study aims to evaluate the mutagenicity of multi-walled carbon nanotubes (MWCNTs) functionalized in somatic cells of Drosophila melanogaster, using the somatic mutation and recombination test (SMART). This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the wings of the fly. Larvae of three days were used, resulting from ST cross, with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross). They were treated with different concentrations of MWCNTs functionalized. The MH descendants, analyzed in both ST and HB crosses, had no significant effects on the frequency of mutant. Based on the results and on the experimental conditions mentioned in this study, it was concluded that MWCNTs were not mutagenic in D. melanogaster.
碳纳米管（CNTs）是通过将单个石墨片卷成管状而形成的。在不同类型的碳纳米管中，多壁碳纳米管（MWCNTs）由一组结构完美的同心纳米管组成。多壁碳纳米管的几种用途已被建议应用于生物领域，如制造生物传感器、药物载体等。然而，在这些材料能够投放市场之前，有必要了解它们的遗传毒性作用。因此，本研究旨在利用体细胞突变和重组试验（SMART）评估功能化多壁碳纳米管（MWCNTs）对黑腹果蝇体细胞的致突变性。该试验检测在果蝇翅膀上表型表达的标记基因的杂合性丧失。使用了由ST杂交产生的三天龄幼虫，其细胞色素P450水平处于基础水平，以及具有高代谢生物活性能力的幼虫（HB杂交）。它们用不同浓度的功能化多壁碳纳米管进行处理。在ST和HB杂交中分析的MH后代对突变频率没有显著影响。根据本研究的结果和实验条件，得出结论：多壁碳纳米管对黑腹果蝇没有致突变性。
