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四种3'-5' RNA聚合酶不同生物学功能的鉴定。

Identification of distinct biological functions for four 3'-5' RNA polymerases.

作者信息

Long Yicheng, Abad Maria G, Olson Erik D, Carrillo Elisabeth Y, Jackman Jane E

机构信息

Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA.

Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.

出版信息

Nucleic Acids Res. 2016 Sep 30;44(17):8395-406. doi: 10.1093/nar/gkw681. Epub 2016 Aug 2.

DOI:10.1093/nar/gkw681
PMID:27484477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5041481/
Abstract

The superfamily of 3'-5' polymerases synthesize RNA in the opposite direction to all other DNA/RNA polymerases, and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well as Thg1-like proteins (TLPs) of unknown function that are broadly distributed, with family members in all three domains of life. Dictyostelium discoideum encodes one Thg1 and three TLPs (DdiTLP2, DdiTLP3 and DdiTLP4). Here, we demonstrate that depletion of each of the genes results in a significant growth defect, and that each protein catalyzes a unique biological reaction, taking advantage of specialized biochemical properties. DdiTLP2 catalyzes a mitochondria-specific tRNA(His) maturation reaction, which is distinct from the tRNA(His) maturation reaction typically catalyzed by Thg1 enzymes on cytosolic tRNA. DdiTLP3 catalyzes tRNA repair during mitochondrial tRNA 5'-editing in vivo and in vitro, establishing template-dependent 3'-5' polymerase activity of TLPs as a bona fide biological activity for the first time since its unexpected discovery more than a decade ago. DdiTLP4 is cytosolic and, surprisingly, catalyzes robust 3'-5' polymerase activity on non-tRNA substrates, strongly implying further roles for TLP 3'-5' polymerases in eukaryotes.

摘要

3'-5'聚合酶超家族以与所有其他DNA/RNA聚合酶相反的方向合成RNA，其成员包括真核生物的tRNA(His)鸟苷酸转移酶（Thg1），以及功能未知的Thg1样蛋白（TLP），这些蛋白广泛分布于生命的三个域中的各个家族成员中。盘基网柄菌编码一种Thg1和三种TLP（DdiTLP2、DdiTLP3和DdiTLP4）。在这里，我们证明每个基因的缺失都会导致显著的生长缺陷，并且每种蛋白质利用特殊的生化特性催化独特的生物学反应。DdiTLP2催化线粒体特异性的tRNA(His)成熟反应，这与Thg1酶通常在胞质tRNA上催化的tRNA(His)成熟反应不同。DdiTLP3在体内和体外催化线粒体tRNA 5'-编辑过程中的tRNA修复，自十多年前意外发现以来，首次确立了TLP的模板依赖性3'-5'聚合酶活性作为一种真正的生物学活性。DdiTLP4位于胞质中，令人惊讶的是，它在非tRNA底物上催化强大的3'-5'聚合酶活性，这强烈暗示了TLP 3'-5'聚合酶在真核生物中的进一步作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/1b5b84309aad/gkw681fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/607c3a79c7ad/gkw681fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/094b2e3f4768/gkw681fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/7b60aea20717/gkw681fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/892bd263b136/gkw681fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/369cb7076fef/gkw681fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/4c7338a19003/gkw681fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/b0e3bab25f93/gkw681fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/1b5b84309aad/gkw681fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/607c3a79c7ad/gkw681fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/094b2e3f4768/gkw681fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/7b60aea20717/gkw681fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/892bd263b136/gkw681fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/369cb7076fef/gkw681fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/4c7338a19003/gkw681fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/b0e3bab25f93/gkw681fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b356/5041481/1b5b84309aad/gkw681fig8.jpg

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Int J Mol Sci. 2014 Dec 22;15(12):23975-98. doi: 10.3390/ijms151223975.
2
Life without post-transcriptional addition of G-1: two alternatives for tRNAHis identity in Eukarya.没有转录后添加 G-1：真核生物中 tRNAHis 身份的两种选择。
RNA. 2015 Feb;21(2):243-53. doi: 10.1261/rna.048389.114. Epub 2014 Dec 12.
3
Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.
RNA. 2024 Sep 16;30(10):1315-1327. doi: 10.1261/rna.080156.124.
4
The life and times of a tRNA.tRNA 的一生和时代。
RNA. 2023 Jul;29(7):898-957. doi: 10.1261/rna.079620.123. Epub 2023 Apr 13.
5
Fidelity of base-pair recognition by a 3'-5' polymerase: mechanism of the tRNA guanylyltransferase.3'-5'聚合酶对碱基对识别的保真度：tRNA 鸟苷酰转移酶的机制。
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6
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RNA. 2021 Jun;27(6):665-675. doi: 10.1261/rna.078287.120. Epub 2021 Mar 23.
7
Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in .真核生物 CCA-添加酶的异常发生。
Int J Mol Sci. 2020 Jul 23;21(15):5210. doi: 10.3390/ijms21155210.
8
Naturally occurring dual recognition of tRNA substrates with and without a universal identity element.天然存在的 tRNA 底物的双重识别，具有和不具有通用识别元件。
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