牛和人肾上腺中胆固醇侧链裂解细胞色素P-450的免疫组织化学显示

Immunohistochemical demonstration of cholesterol side-chain cleavage cytochrome P-450 in bovine and human adrenals.

作者信息

Sasano H, Sasano N, Okamoto M

机构信息

Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Pathol Res Pract. 1989 Mar;184(3):337-42. doi: 10.1016/S0344-0338(89)80096-2.

DOI:10.1016/S0344-0338(89)80096-2

PMID:2748459

Abstract

Cytochrome P-450 specific for cholesterol side-chain cleavage (P-450SCC) was purified from the bovine adrenal and a specific antibody was raised in rabbits. The antiserum was applied for immunohistochemical visualization of the P-450SCC in the bovine and human adrenal cortex. The immunoreactivity was intense in the zona fasciculata (ZF) and reticularis (ZR) while weak in the zona glomerulosa (ZG) in the normal adrenals. In adrenocortical hyperplasia, a marked immunoreactivity was observed in the ZG in idiopathic hyperaldosteronism and the inner ZF and reticularis particularly in cells of micronodules in Cushing's disease, corresponding to cells with active steroidogenesis. In aldosteronoma and adenoma with Cushing's syndrome, P-450SCC was generally present in compact cells of adenomas.

摘要

从牛肾上腺中纯化出胆固醇侧链裂解特异性细胞色素P-450（P-450SCC），并在兔体内制备了特异性抗体。将抗血清用于牛和人肾上腺皮质中P-450SCC的免疫组织化学可视化。在正常肾上腺中，束状带（ZF）和网状带（ZR）的免疫反应强烈，而球状带（ZG）的免疫反应较弱。在肾上腺皮质增生中，特发性醛固酮增多症的ZG中观察到明显的免疫反应，库欣病的ZF内层和网状带，特别是微结节细胞中，免疫反应明显，对应于具有活跃类固醇生成的细胞。在醛固酮瘤和库欣综合征腺瘤中，P-450SCC通常存在于腺瘤的致密细胞中。