Sasano H, Sasano N, Okamoto M, Nonaka Y
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Pathol Res Pract. 1989 May;184(5):473-9. doi: 10.1016/S0344-0338(89)80137-2.
Adrenodoxin reductase (ADR) was purified from bovine adrenocortical mitochondria and specific antibody was raised in rabbits. Immunohistochemical analysis of ADR was performed in the bovine and human adrenals. ADR was present in all of the zones in both bovine and non-pathological human adrenal cortex. In non-pathological human adrenals, the immunoreactivity was particularly prominent in the zona glomerulosa (ZG) and reticularis (ZR). Intensive immunoreactivity was observed in the ZG and some cells of the outer fasciculata and the ZR in the adrenal glands with idiopathic hyperaldosteronism. In adrenal glands with Cushing's disease, immunoreactivity was present in the compact cells of cortical micronodules. In all cases, sites of immunoreactivity correspond to sites of increased steroidogenesis. In aldosteronoma and cortical adenoma with Cushing's syndrome, the immunoreactivity was generally marked in compact cells but not in large cells with clear cytoplasm, ADR was present in the ZG and the ZR, and the ZG in the non-neoplastic adrenal glands attached to aldosteronoma and Cushing's adenoma, respectively. ADR was present in the compact cells in adrenocortical carcinoma clinically manifesting Cushing's syndrome.
从牛肾上腺皮质线粒体中纯化出肾上腺皮质铁氧化还原蛋白还原酶（ADR），并在兔体内制备了特异性抗体。对牛和人的肾上腺进行了ADR的免疫组织化学分析。ADR存在于牛和非病理性人肾上腺皮质的所有区域。在非病理性人肾上腺中，免疫反应性在球状带（ZG）和网状带（ZR）中尤为突出。在特发性醛固酮增多症患者的肾上腺中，在ZG以及束状带外层的一些细胞和ZR中观察到强烈的免疫反应性。在库欣病患者的肾上腺中，皮质微小结节的致密细胞中存在免疫反应性。在所有病例中，免疫反应部位与类固醇生成增加的部位相对应。在醛固酮瘤和库欣综合征的皮质腺瘤中，致密细胞中的免疫反应性通常很明显，但在细胞质清晰的大细胞中则不明显，ADR分别存在于与醛固酮瘤和库欣腺瘤相连的非肿瘤性肾上腺的ZG和ZR中。ADR存在于临床诊断为库欣综合征的肾上腺皮质癌的致密细胞中。
