Sasano H, Mason J I, Sasano N
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Hum Pathol. 1989 Feb;20(2):113-7. doi: 10.1016/0046-8177(89)90174-3.
Cytochrome P-450 specific for steroid 17 alpha-hydroxylation (P-45017 alpha) was immunolocalized in normal and hyperfunctioning adrenal glands of pigs, bovines, and humans, using a specific IgG fraction raised against the enzyme. P-45017 alpha was present in the zona fasciculata (ZF) and zona reticularis (ZR), but not in the zona glomerulosa (ZG), in pig, bovine, and human adrenal glands. In the adrenal glands of patients with Cushing's disease, the positive immunoreactivity to P-45017 alpha was intense in ZF and ZR, particularly in cortical micronodules, corresponding to the sites of active steroidogenesis. Cells of hyperplastic ZG and outer ZF in the adrenal glands of idiopathic hyperaldosteronism were negative for P-45017 alpha. In aldosteronoma, positive immunoreactivity was observed in some tumor cells, which is consistent with cortisol production and its responsiveness to ACTH in aldosteronoma. In the attached adrenal glands of aldosteronoma, the immunoreactive P-45017 alpha was clearly present in the inner ZF and ZR, suggesting persistent androgen production. In Cushing's adenoma, the positive immunoreactivity was intense in tumor cells, and the ZR of the attached adrenal glands was weakly immunoreactive.
利用针对类固醇17α-羟化酶（P-45017α）产生的特异性IgG组分，对猪、牛和人的正常及功能亢进肾上腺中的细胞色素P-450（特异性针对类固醇17α-羟化）进行免疫定位。在猪、牛和人的肾上腺中，P-45017α存在于束状带（ZF）和网状带（ZR），但不存在于球状带（ZG）。在库欣病患者的肾上腺中，ZF和ZR对P-45017α的阳性免疫反应强烈，尤其是在皮质微小结节中，对应于活跃的类固醇生成部位。特发性醛固酮增多症患者肾上腺中增生的ZG和外层ZF细胞对P-45017α呈阴性。在醛固酮瘤中，在一些肿瘤细胞中观察到阳性免疫反应，这与醛固酮瘤中皮质醇的产生及其对促肾上腺皮质激素的反应性一致。在醛固酮瘤的附属肾上腺中，免疫反应性P-45017α明显存在于内层ZF和ZR中，提示雄激素持续产生。在库欣腺瘤中，肿瘤细胞中的阳性免疫反应强烈，附属肾上腺的ZR呈弱免疫反应。
