Kim Hyun-Duck, Shin Myung-Seop, Kim Hyun-Tae, Kim Mi-Sun, Ahn Yoo-Been
Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University, Seoul, Korea.
Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
J Clin Periodontol. 2016 Dec;43(12):1032-1040. doi: 10.1111/jcpe.12607. Epub 2016 Oct 3.
This study aims to evaluate the association of salivary matrix metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8) with periodontitis and its screening ability on periodontitis.
We selected 693 participants from the Yanypyeong Cohort: 577 participants with periodontitis and 116 participants without periodontitis. Periodontitis was assessed by dentists using a panoramic radiograph. Salivary MMP-9 and IL-8 were assayed using multiplexed bead immunoassay (Luminex). MMP-9 and IL-8 were categorized into low, medium and high. Age, sex, income, smoking, drinking, exercise, obesity and metabolic syndrome were confounders. Logistic regression analysis was applied to evaluate the association between MMP-9, IL-8 and periodontitis. Receiver operating characteristic curve was applied for sensitivity, specificity and c-statistics.
High MMP-9 and medium IL-8 were associated with periodontitis: adjusted odds ratio were 2.2 [95% confidence interval (CI): 1.3-3.7] for MMP-9 and 1.9 (95% CI: 1.1-3.4) for IL-8. The final screening model using salivary MMP-9 for periodontitis had a sensitivity of 0.46, specificity of 0.77 and c-statistic of 0.63 (p < 0.001).
Our data show that salivary MMP-9 and IL-8 could be potential markers for periodontitis. The screening model for periodontitis could be useful in clinics and home. A future prospective study is indicated for predicting the occurrence of periodontitis.
本研究旨在评估唾液基质金属蛋白酶-9（MMP-9）和白细胞介素-8（IL-8）与牙周炎的关联及其对牙周炎的筛查能力。
我们从牙平队列中选取了693名参与者：577名患有牙周炎的参与者和116名未患牙周炎的参与者。牙医使用全景X线片评估牙周炎。使用多重微珠免疫测定法（Luminex）检测唾液中的MMP-9和IL-8。MMP-9和IL-8被分为低、中、高三个等级。年龄、性别、收入、吸烟、饮酒、运动、肥胖和代谢综合征为混杂因素。应用逻辑回归分析评估MMP-9、IL-8与牙周炎之间的关联。应用受试者工作特征曲线评估敏感性、特异性和c统计量。
高MMP-9和中等水平的IL-8与牙周炎相关：MMP-9的调整比值比为2.2 [95%置信区间（CI）：1.3 - 3.7]，IL-8的调整比值比为1.9（95% CI：1.1 - 3.4）。使用唾液MMP-9筛查牙周炎的最终模型的敏感性为0.46，特异性为0.77，c统计量为0.63（p < 0.001）。
我们的数据表明，唾液MMP-9和IL-8可能是牙周炎的潜在标志物。牙周炎筛查模型在临床和家庭中可能有用。未来需要进行前瞻性研究以预测牙周炎的发生。
