Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University, Seoul, Korea.
Dental Research Institute, Seoul National University, Seoul, Korea.
J Clin Periodontol. 2020 Oct;47(10):1191-1200. doi: 10.1111/jcpe.13349. Epub 2020 Sep 15.
Salivary diagnostic using matrix metalloproteinase (MMP) and S100 for periodontitis is a promising issue. However, its prognostic effect is still unclear. This study aimed to evaluate the prognostic ability of salivary MMP-9 and S100A8 for periodontitis through non-surgical periodontitis treatment clinical trial.
Total 149 participants, 99 periodontitis and 50 healthy, were recruited. Among 99 non-surgical periodontitis treatment participants, 74 participants were revisited after three months. Periodontitis was classified as stage II-IV of new classification of periodontitis proposed at 2018. Enzyme-linked immunosorbent assay kit was used to quantify salivary MMP-9 and S100A8. Receiver operating characteristic curve was applied for diagnostic ability. Paired t test was applied for prognostic ability evaluating changes in salivary markers between pre- and post-treatment.
Salivary MMP-9 and S100A8 were associated with periodontitis (p < .05). The screening ability of algorithm using salivary MMP-9 and S100A8 for periodontitis was 0.86 (p < .05). After treatment, reduction rate of salivary S100A8 and MMP-9 was 83.7% and 23.5%, respectively, (p < .05): only salivary S100A8 was superior compared to clinical parameters.
Algorithm using salivary MMP-9 and S100A8 showed high diagnostic power for periodontitis. Both salivary S100A8 and MMP-9 showed prognostic ability for periodontitis, but S100A8 was better.
利用基质金属蛋白酶（MMP）和 S100 进行唾液诊断在牙周炎中是一个很有前途的问题。然而，其预后效果仍不清楚。本研究旨在通过非手术牙周炎治疗临床试验评估唾液 MMP-9 和 S100A8 对牙周炎的预后能力。
共招募了 149 名参与者，其中 99 名为牙周炎患者，50 名为健康对照者。在 99 名接受非手术牙周炎治疗的参与者中，有 74 名在三个月后进行了复查。牙周炎根据 2018 年提出的牙周炎新分类标准分为 II-IV 期。酶联免疫吸附测定试剂盒用于定量唾液 MMP-9 和 S100A8。应用受试者工作特征曲线评估诊断能力。配对 t 检验用于评估治疗前后唾液标志物变化的预后能力。
唾液 MMP-9 和 S100A8 与牙周炎有关（p<0.05）。使用唾液 MMP-9 和 S100A8 的算法对牙周炎的筛查能力为 0.86（p<0.05）。治疗后，唾液 S100A8 和 MMP-9 的降低率分别为 83.7%和 23.5%（p<0.05）：仅唾液 S100A8 优于临床参数。
使用唾液 MMP-9 和 S100A8 的算法对牙周炎具有较高的诊断能力。唾液 S100A8 和 MMP-9 均对牙周炎具有预后能力，但 S100A8 更好。
