Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University, Seoul, Korea.
Dental Research Institute, Seoul National University, Seoul, Korea.
J Clin Periodontol. 2020 Nov;47(11):1354-1361. doi: 10.1111/jcpe.13360.
This cross-sectional study aimed to examine the diagnostic ability of salivary matrix metalloproteinase (MMP)-9 lateral flow test (LFT) point-of-care (POC) kit and develop an algorithm for diagnosis of periodontitis.
Through Seoul National Dental Hospital, 137 participants (46 LFT negatives, 91 LFT positives) were recruited. For salivary diagnostics, 150 μl of the unstimulated saliva was applied to LFT-POC kit. To make a diagnosis of periodontitis, stage II-IV in modified new international classification system was used. Covariates encompassing age, sex, smoking and obesity were evaluated through face-to-face interview. Enzyme-linked immunosorbent assay was used for quantification of salivary MMP-9. To develop a diagnostic algorithm, multivariable logistic regression analysis was used. Receiver operating characteristic curve was applied for evaluating diagnostic ability.
Diagnostic ability of salivary MMP-9 LFT-POC test was 0.82 (sensitivity of 0.92, specificity of 0.72) in total participants. Diagnostic algorithm using POC test resulted in a response equation, that is algorithm score = -3.675 + 2.877LFT + 0.034age + 0.121sex + 0.372smoking + 0.192*obesity. Diagnostic ability of the algorithm was 0.88 (sensitivity of 0.92, specificity of 0.85) with cut-off score of 0.589.
Salivary MMP-9 LFT-POC kit showed appropriate diagnostic ability for periodontitis and would be an efficient tool for screening of periodontitis.
本横断面研究旨在检验唾液基质金属蛋白酶（MMP）-9 侧向流动检测（LFT）即时检测（POC）试剂盒的诊断能力，并开发一种用于牙周炎诊断的算法。
通过首尔国立牙科医院，招募了 137 名参与者（46 名 LFT 阴性，91 名 LFT 阳性）。对于唾液诊断，将 150μl 未刺激唾液应用于 LFT-POC 试剂盒。使用改良的新国际分类系统的 II-IV 期来做出牙周炎的诊断。通过面对面访谈评估包含年龄、性别、吸烟和肥胖等变量。使用酶联免疫吸附测定法定量唾液 MMP-9。为了开发诊断算法，使用多变量逻辑回归分析。应用受试者工作特征曲线评估诊断能力。
在所有参与者中，唾液 MMP-9 LFT-POC 检测的诊断能力为 0.82（敏感性为 0.92，特异性为 0.72）。使用 POC 检测的诊断算法得出了一个响应方程，即算法评分=-3.675+2.877LFT+0.034年龄+0.121性别+0.372吸烟+0.192*肥胖。算法的诊断能力为 0.88（敏感性为 0.92，特异性为 0.85），截断值为 0.589。
唾液 MMP-9 LFT-POC 试剂盒对牙周炎具有适当的诊断能力，将成为牙周炎筛查的有效工具。
