Vaisberg A J, Milla M, Planas M C, Cordova J L, de Agusti E R, Ferreyra R, Mustiga M C, Carlin L, Hammond G B
Planta Med. 1989 Apr;55(2):140-3. doi: 10.1055/s-2006-961907.
Sangre de Grado extract used by Peruvian natives as a cicatrizant agent, was collected from trees of the species Croton lechleri growing in the Peruvian jungle. The Sangre de Grado was found to contain one alkaloid identified as taspine and which was shown to be the active cicatrizant principle by an in vivo test in mice. This alkaloid exhibited a dose-related cicatrizant effect and an ED50 of 0.375 mg/kg. Experiments with taspine hydrochloride in order to study its mechanism of action in cell culture systems showed that the alkaloid was non-toxic to human foreskin fibroblasts at concentrations below 150 ng/ml and that it had no effect on cell proliferation. On the other hand, taspine hydrochloride was found to increase the migration of human foreskin fibroblasts. This effect on the migration of fibroblasts is probably the mechanism by which Sangre de Grado and taspine hydrochloride accelerate the wound healing process. Using the two-stage mouse skin carcinogenesis system, we have been able to show that neither Sangre de Grado nor taspine hydrochloride had carcinogenic or tumour promoter activity after 17 months of treatment.
秘鲁当地人用作愈合剂的血根提取物，是从生长在秘鲁丛林中的巴豆属植物（Croton lechleri）的树上采集的。发现血根含有一种被鉴定为塔斯品碱的生物碱，通过在小鼠体内进行的试验表明它是活性愈合成分。这种生物碱呈现出剂量相关的愈合作用，半数有效剂量为0.375毫克/千克。用盐酸塔斯品碱进行实验以研究其在细胞培养系统中的作用机制，结果表明该生物碱在浓度低于150纳克/毫升时对人包皮成纤维细胞无毒，并且对细胞增殖没有影响。另一方面，发现盐酸塔斯品碱可增加人包皮成纤维细胞的迁移。对成纤维细胞迁移的这种作用可能是血根和盐酸塔斯品碱加速伤口愈合过程的机制。使用两阶段小鼠皮肤致癌系统，我们已经能够证明，在治疗17个月后，血根和盐酸塔斯品碱均无致癌或肿瘤促进活性。
