Bakhshi Poonam K, Bain Jennifer, Gul Mine Orlu, Stride Eleanor, Edirisinghe Mohan, Staniland Sarah S
Department of Mechanical Engineering, University College London, Roberts, Torrington Place, London, WC1E 7JE, UK.
Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK.
Macromol Biosci. 2016 Nov;16(11):1555-1561. doi: 10.1002/mabi.201600181. Epub 2016 Aug 4.
A new synthetic method for the production of artificial magnetosomes, i.e., lipid-coated vesicles containing magnetic nanoparticles, is demonstrated. Magnetosomes have considerable potential in biomedical and other nanotechnological applications but current production methods rely upon magnetotactic bacteria which limits the range of sizes and shapes that can be generated as well as the obtainable yield. Here, electrohydrodynamic atomization is utilized to form nanoscale liposomes of tunable size followed by electroporation to transport iron into the nanoliposome core resulting in magnetite crystallization. Using a combination of electron and fluorescence microscopy, dynamic light scattering, Raman spectroscopy, and magnetic susceptibility measurements, it is shown that single crystals of single-phase magnetite can be precipitated within each liposome, forming a near-monodisperse population of magnetic nanoparticles. For the specific conditions used in this study the mean particle size is 58 nm (±8 nm) but the system offers a high degree of flexibility in terms of both the size and composition of the final product.
展示了一种生产人工磁小体的新合成方法，即含有磁性纳米颗粒的脂质包被囊泡。磁小体在生物医学和其他纳米技术应用中具有巨大潜力，但目前的生产方法依赖趋磁细菌，这限制了可生成的尺寸和形状范围以及可获得的产量。在此，利用电流体动力学雾化形成尺寸可调的纳米级脂质体，随后通过电穿孔将铁转运到纳米脂质体核心中，从而导致磁铁矿结晶。通过结合电子显微镜和荧光显微镜、动态光散射、拉曼光谱以及磁化率测量，结果表明单相磁铁矿的单晶可在每个脂质体内沉淀，形成近乎单分散的磁性纳米颗粒群体。对于本研究中使用的特定条件，平均粒径为58纳米（±8纳米），但该系统在最终产品的尺寸和组成方面具有高度灵活性。
