Park Hyunwoo, Weier Steven, Razvi Fareha, Peña Pamela A, Sims Neil A, Lowell Jennica, Hungate Cory, Kissinger Karma, Key Gavin, Fraser Paul, Napier Johnathan A, Cahoon Edgar B, Clemente Tom E
Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
Plant Biotechnol J. 2017 Feb;15(2):227-236. doi: 10.1111/pbi.12608. Epub 2016 Sep 13.
Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega-3 fatty acids in harvested flesh. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity-preserved soya bean-based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value-added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.
大豆（Glycine max (L.) Merr.）因其油脂和蛋白质成分而备受青睐。在大豆育种和分子生物学项目中，通过基因方法提高这两种成分的价值是正在进行的工作。前者是全球消费的主要植物油。因此，其主要用途是直接供人类食用。为了提高其在饲料应用中的效用，从而扩大大豆副产品市场，我们研究了在虹鳟（Oncorhynchus mykiss）和五条鰤（Seriola rivoliana）的水产饲料中，用大豆基蛋白和富含α-亚麻酸和十八碳四烯酸的高欧米伽-3脂肪酸大豆油同时替代海洋成分。本文介绍了水产饲料配方，其中海洋成分大幅减少，这意味着收获的鱼肉中总欧米伽-3脂肪酸含量更高。基于这些发现，后续工作转向了一种基因策略，该策略将转化为一种用于水产养殖的基于大豆的最佳身份保留原料的原型设计，即通过多基因叠加方法靶向合成两种增值产出性状——二十碳五烯酸和酮类胡萝卜素虾青素，并将其引入作物。为此，本文描述了将七个转基因盒系统地引入大豆以及对所得新事件的分子和表型评估。
