Tan N H, Hj M N
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Toxicon. 1989;27(6):689-95. doi: 10.1016/0041-0101(89)90020-2.
Some enzymatic activities and toxic properties of four samples of Ophiophagus hannah (king cobra) venom were investigated. There is little intraspecific variation in enzyme contents, protein composition and toxic properties of the venom. The venom does not exhibit hemolytic or edema-inducing activity but is characterized by an exceptionally high alkaline phosphomonoesterase activity. DEAE-Sephacel ion exchange chromatography and Sephadex G-75 gel filtration chromatography of the venom indicate that the major lethal toxins are the low mol.wt, non-enzymatic basic proteins. Venom fractions exhibiting high enzymatic activities apparently do not play an important role in the lethality in mice of Ophiophagus hannah venom.
对四个眼镜王蛇样本的毒液进行了一些酶活性和毒性特性研究。该毒液在酶含量、蛋白质组成及毒性特性方面种内差异很小。该毒液不表现出溶血或致水肿活性，但其特征是具有异常高的碱性磷酸单酯酶活性。对该毒液进行的二乙氨基乙基纤维素离子交换色谱法和葡聚糖凝胶G-75凝胶过滤色谱法表明，主要致死毒素是低分子量的非酶碱性蛋白。表现出高酶活性的毒液组分显然在眼镜王蛇毒液对小鼠的致死性方面不起重要作用。
