Redman C M, Lee S, ten Huinink D, Rabin B I, Johnson C L, Oyen R, Marsh W L
Lindsley F. Kimball Research Institute, New York Blood Center.
Transfusion. 1989 Jul-Aug;29(6):486-90. doi: 10.1046/j.1537-2995.1989.29689318444.x.
Kell antigens on chimpanzee (Pan troglodytes) red cells were determined using specific human alloimmune and murine monoclonal antibodies. After avoidance of interspecies reactions, chimpanzee red cells were found to react with most Kell system antibodies. The chimpanzees had phenotypes similar to those of humans. The main difference was that all of 27 chimpanzee red cell samples tested were of the K:6, -7, phenotype, while in humans most are K:-6, 7. The most common chimpanzee Kell blood group phenotype was K:-1,2,-3,4,5,6,-7,11,12,13,14, 15,18,19,22. Murine monoclonal anti-K2 and -K14 immunoprecipitated a 97-kD protein from chimpanzee red cells and a 93-kD protein from human red cells. Enzymatic deglycosylation yielded proteins of about 79 kD for humans and 77 kD for chimpanzees. Both human and chimpanzee Kell proteins reacted equally well on Western blots with polyclonal rabbit antibody to human Kell protein, which indicated close homology.
利用特异性人类同种免疫抗体和鼠单克隆抗体对黑猩猩（Pan troglodytes）红细胞上的凯尔抗原进行了测定。在避免种间反应后，发现黑猩猩红细胞能与大多数凯尔系统抗体发生反应。黑猩猩的血型表型与人类相似。主要区别在于，所检测的27份黑猩猩红细胞样本均为K:6, -7表型，而人类大多数为K:-6, 7表型。最常见的黑猩猩凯尔血型表型为K:-1,2,-3,4,5,6,-7,11,12,13,14, 15,18,19,22。鼠单克隆抗K2和抗K14从黑猩猩红细胞中免疫沉淀出一种97-kD蛋白，从人类红细胞中免疫沉淀出一种93-kD蛋白。酶促去糖基化反应使人类产生约79 kD的蛋白，黑猩猩产生约77 kD的蛋白。在蛋白质印迹实验中，人类和黑猩猩的凯尔蛋白与兔抗人凯尔蛋白多克隆抗体反应同样良好，这表明它们具有密切的同源性。
