斑点热群立克次体菌株在Vero细胞中的生长比较

Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells.

作者信息

Silva Arannadia Barbosa, Duarte Myrian Morato, Vizzoni Vinicius Figueiredo, Duré Ana Íris de Lima, Lopéz Diego Montenegro, Nogueira Rita de Maria Seabra, Soares Carlos Augusto Gomes, Machado-Ferreira Erik, Gazêta Gilberto Salles

机构信息

Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Programa de Pós-Graduação em Biodiversidade e Saúde, Rio de Janeiro, RJ, Brasil.

Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Referência Nacional em Vetores das Riquetsioses, Rio de Janeiro, RJ, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2016 Aug;111(8):528-31. doi: 10.1590/0074-02760160093.

DOI:10.1590/0074-02760160093

PMID:27508322

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4981112/

Abstract

In Brazil, the spotted fever group (SFG) Rickettsia rickettsii and Rickettsia parkeri related species are the etiological agents of spotted fever rickettsiosis. However, the SFG, Rickettsia rhipicephali, that infects humans, has never been reported. The study of growth dynamics can be useful for understanding the infective and invasive capacity of these pathogens. Here, the growth rates of the Brazilian isolates R. rickettsii str. Taiaçu, R. parkeri str. At#24, and R. rhipicephali HJ#5, were evaluated in Vero cells by quantitative polymerase chain reaction. R. rhipicephali showed different kinetic growth compared to R. rickettsii and R. parkeri.

摘要

在巴西，斑点热群（SFG）立克次体和与帕克立克次体相关的物种是斑点热立克次体病的病原体。然而，感染人类的SFG立克次体（Rickettsia rhipicephali）从未有过报道。生长动力学的研究有助于理解这些病原体的感染和侵袭能力。在此，通过定量聚合酶链反应在Vero细胞中评估了巴西分离株立氏立克次体（R. rickettsii）Taiaçu株、帕克立克次体（R. parkeri）At#24株和头蜱立克次体（R. rhipicephali）HJ#5株的生长速率。与立氏立克次体和帕克立克次体相比，头蜱立克次体呈现出不同的生长动力学。