Maddalo Danilo, Ventura Andrea
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York.
Cancer Res. 2016 Sep 1;76(17):4918-23. doi: 10.1158/0008-5472.CAN-16-0726. Epub 2016 Aug 12.
The ability to engineer specific mutations in mice has proven essential to advancing our understanding of the molecular basis of cancer. Chromosomal rearrangements, a common and clinically relevant class of cancer-causing mutations, have however remained difficult to faithfully recapitulate in vivo The development of genetic tools for in vivo somatic genome editing has recently overcome this limitation and led to the generation of more sophisticated and accurate preclinical models of human cancers. Here, we review the potential applications of these new technologies to the study of tumor biology and discuss their advantages over more conventional strategies, their limitations, and the remaining challenges. Cancer Res; 76(17); 4918-23. ©2016 AACR.
事实证明，在小鼠中设计特定突变的能力对于推动我们对癌症分子基础的理解至关重要。然而，染色体重排作为一类常见且与临床相关的致癌突变，在体内仍难以如实地重现。用于体内体细胞基因组编辑的遗传工具的发展最近克服了这一限制，并导致生成了更复杂、准确的人类癌症临床前模型。在这里，我们回顾这些新技术在肿瘤生物学研究中的潜在应用，并讨论它们相对于更传统策略的优势、局限性以及 remaining challenges。《癌症研究》；76(17)；4918 - 23。©2016美国癌症研究协会。
需注意，原文中“remaining challenges”未翻译，可能是原文此处有误，正常应为“remaining challenges”的完整翻译“剩余挑战” 。
