Dow Lukas E
Department of Medicine, Hematology and Medical Oncology, Weill Cornell Medical College, New York, NY 10021, USA.
Trends Mol Med. 2015 Oct;21(10):609-621. doi: 10.1016/j.molmed.2015.07.006.
The recent advent of CRISPR/Cas9-mediated genome editing has created a wave of excitement across the scientific research community, carrying the promise of simple and effective genomic manipulation of nearly any cell type. CRISPR has quickly become the preferred tool for genetic manipulation, and shows incredible promise as a platform for studying gene function in vivo. I discuss the current application of CRISPR technology to create new in vivo disease models, with a particular focus on how these tools, derived from an adaptive bacterial immune system, are helping us to better model the complexity of human cancer.
CRISPR/Cas9介导的基因组编辑技术的近期出现，在整个科研界掀起了一阵热潮，有望对几乎任何细胞类型进行简单有效的基因组操作。CRISPR迅速成为基因操作的首选工具，并作为一种在体内研究基因功能的平台展现出了令人难以置信的前景。我将讨论CRISPR技术目前在创建新的体内疾病模型方面的应用，特别关注这些源自适应性细菌免疫系统的工具如何帮助我们更好地模拟人类癌症的复杂性。
