Microbial Ecology Laboratory, Department of Applied Biosciences, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India.
Int J Syst Evol Microbiol. 2018 Sep;68(9):2969-2976. doi: 10.1099/ijsem.0.002931. Epub 2018 Jul 20.
A novel Gram-stain-variable, rod-shaped, non-motile and non-endospore-forming bacterium (strain G27) was isolated from near Dhuvaran, Gujarat, India. Based on 16S rRNA gene sequence analysis, strain G27 was identified as a member of the class Firmibacteria and was most closely related to Bacillus populi FJAT-45347 (94.9 % sequence similarity), Salipaludibacillus aurantiacus S9 (94.9 %), Salipaludibacillus neizhouensis KCTC 13187 (94.7 %), Alteribacillus iranensis DSM 23995 (94.6 %) and other Firmibacteria (<94.6 %). The DNA G+C content of strain G27 was 43.4±0.6 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids. The predominant isoprenoid quinone was menaquinone MK-7. Major fatty acids (>5 %) included anteiso-C15:0, iso-C15 : 0, anteiso-C17:0, C16 : 0 and iso-C16 : 0. The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain G27 from all other members of the family Bacillaceae.It is therefore considered to represent a novel species of a new genus, for which the name Thalassorhabdus alkalitolerans gen. nov., sp. nov., is proposed. The type strain of Thalassorhabdus alkalitolerans is G27 (=MCC 3411=CGMCC 1.15772=KCTC 33941).
从印度古吉拉特邦的杜瓦尔地区附近分离到一株革兰氏染色可变、杆状、非运动和非内生孢子形成的细菌（菌株 G27）。基于 16S rRNA 基因序列分析，菌株 G27 被鉴定为厚壁菌门的一个成员，与杨树芽孢杆菌 FJAT-45347（94.9%序列相似性）、橙黄沙雷氏菌 S9（94.9%）、内黄沙雷氏菌 KCTC 13187（94.7%）、伊朗交替单胞菌 DSM 23995（94.6%）和其他厚壁菌门（<94.6%）最为密切相关。菌株 G27 的 DNA G+C 含量为 43.4±0.6mol%。细胞壁肽聚糖含有 meso-二氨基庚二酸。极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和五种未鉴定的脂类。主要的异戊烯醌是menaquinone MK-7。主要脂肪酸（>5%）包括 anteiso-C15:0、iso-C15:0、anteiso-C17:0、C16:0 和 iso-C16:0。系统发育、化学分类和生化测试的结果允许将菌株 G27 与芽孢杆菌科的所有其他成员清楚地区分开来。因此，它被认为代表了一个新属的一个新种，建议将其命名为耐碱海洋盐单胞菌属 nov. gen.，nov. sp.。耐碱海洋盐单胞菌的模式菌株是 G27（=MCC 3411=CGMCC 1.15772=KCTC 33941）。
