组胺和5-羟色胺抑制鸟氨酸对灌流的艾氏腹水癌细胞中鸟氨酸脱羧酶的诱导作用。

Histamine and serotonin inhibit induction of ornithine decarboxylase by ornithine in perifused Ehrlich ascites tumour cells.

作者信息

Matés J M, Sánchez-Jiménez F M, García-Caballero M, Núñez de Castro I

机构信息

Cátedra de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.

出版信息

FEBS Lett. 1989 Jul 3;250(2):257-61. doi: 10.1016/0014-5793(89)80733-1.

DOI:10.1016/0014-5793(89)80733-1

PMID:2753136

Abstract

Ornithine induced more than 36-fold the ornithine decarboxylase activity in confined Ehrlich ascites tumour cells after 3.5 h of continuous perifusion with 0.5 mM ornithine; arginine and glutamine also induced the activity 3- and 4-fold, respectively. The addition of cycloheximide or actinomycin D antibiotics to the perifusion medium confirmed that the regulation of the enzyme synthesis takes place at the level of translation. Perifusion in the presence of 0.5 mM ornithine and 55, 25, and 10 microM histamine suppressed the induction by 91, 53, and 35% respectively. Similar results were obtained in the presence of serotonin. Histidine also showed inhibitory effect but 5 mM histidine was required to produce 21% inhibition; other basic amino acids were ineffective.

摘要

在对艾氏腹水癌细胞进行连续3.5小时、0.5 mM鸟氨酸的灌注后，鸟氨酸使封闭培养的艾氏腹水癌细胞中的鸟氨酸脱羧酶活性增加了36倍以上；精氨酸和谷氨酰胺也分别使该活性增加了3倍和4倍。向灌注培养基中添加环己酰亚胺或放线菌素D抗生素证实，酶合成的调节发生在翻译水平。在存在0.5 mM鸟氨酸以及55、25和10 microM组胺的情况下进行灌注，分别使诱导作用降低了91%、53%和35%。在血清素存在的情况下也获得了类似结果。组氨酸也显示出抑制作用，但需要5 mM组氨酸才能产生21%的抑制作用；其他碱性氨基酸则无效。