Matés J M, Márquez J, García-Caballero M, Núñez de Castro I, Sánchez-Jiménez F
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Agents Actions. 1992 May;36(1-2):17-21. doi: 10.1007/BF01991222.
A reversed-phase HPLC technique in combination with fluorescent detection is described for simultaneous quantification of the precolumn Dansyl derivatives of intracellular amines. The derivatives were stable for at least one week, kept protected from the light at -20 degrees C. The detection limit was between 1 and 5 pmol for all tested polyamines. Serotonin coeluted with tryptamine. The method has a very good reproducibility for both, retention times and chromatographic peak areas. The average recovery of standard amine solutions added to cellular extracts was estimated to be higher than 90%. The described method enables a rapid, reliable and reproducible quantification of biogenic and related polyamines in biological fluids and tissues.
本文描述了一种结合荧光检测的反相高效液相色谱技术，用于同时定量细胞内胺的柱前丹磺酰衍生物。这些衍生物在 -20℃避光保存时至少稳定一周。所有测试的多胺的检测限在1至5皮摩尔之间。5-羟色胺与色胺共洗脱。该方法对于保留时间和色谱峰面积都具有非常好的重现性。添加到细胞提取物中的标准胺溶液的平均回收率估计高于90%。所描述的方法能够对生物体液和组织中的生物胺及相关多胺进行快速、可靠且可重复的定量。
