Ferbert Thomas, Child Christopher, Graeser Viola, Swing Tyler, Akbar Michael, Heller Raban, Biglari Bahram, Moghaddam Arash
1 HTRG-Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital , Heidelberg, Germany .
2 Berufsgenossenschaftliche Unfallklinik Ludwigshafen , Department of Paraplegiology, Ludwigshafen, Germany .
J Neurotrauma. 2017 Feb;34(3):607-614. doi: 10.1089/neu.2015.4294. Epub 2016 Oct 12.
Neuroinflammation presumably has an important impact on the secondary phase of spinal cord injury and is regulated by pro- and anti-inflammatory cytokines. We analyzed serum levels of three different cytokines (insulin-like-growth-factor [IGF]-1, tumor growth factor [TGF]-β1, and soluble CD 95 ligand [sCD95L]), in blood samples of 23 patients admitted with acute traumatic spinal cord injury between November 2010 and July 2013 with a follow-up period of 12 weeks. Quantification was performed using Human Quantikine Immunoassays, classification of neurological impairment was performed using the American Spinal Cord Injury Impairment Scale at time of admission and after 12 weeks. After an initial drop of all three cytokine serum levels, IGF-1, TGF-β1, and sCD95L showed significantly increased serum levels during the acute and sub-acute phases. For IGF-1 and sCD95L, we could also observe significantly higher serum levels in patients without neurological improvement compared with patients who had improvement after 12 weeks. In this study, we were able to show differences in cytokine serum levels in patients with different neurological outcome. Measuring the serum level patterns of IGF-1, TGF-β1, and sCD95L might be a useful tool for prognosis in patients with neurological improvement and tracking the pathophysiology in further studies. Further, our observations might link promising therapeutic efforts in numerous animal studies and future studies in human patients.
神经炎症可能对脊髓损伤的继发阶段具有重要影响，并受促炎和抗炎细胞因子的调节。我们分析了2010年11月至2013年7月期间收治的23例急性创伤性脊髓损伤患者血液样本中三种不同细胞因子（胰岛素样生长因子[IGF]-1、肿瘤生长因子[TGF]-β1和可溶性CD95配体[sCD95L]）的血清水平，随访期为12周。使用人定量免疫测定法进行定量，入院时和12周后使用美国脊髓损伤损伤量表进行神经功能障碍分类。在所有三种细胞因子血清水平最初下降后，IGF-1、TGF-β1和sCD95L在急性期和亚急性期血清水平显著升高。对于IGF-1和sCD95L，我们还观察到与12周后有改善的患者相比，神经功能无改善的患者血清水平显著更高。在本研究中，我们能够显示不同神经功能转归患者的细胞因子血清水平存在差异。测量IGF-1、TGF-β1和sCD95L的血清水平模式可能是预测神经功能改善患者预后以及在进一步研究中追踪病理生理学的有用工具。此外，我们的观察结果可能将众多动物研究中有前景的治疗方法与未来人类患者研究联系起来。
