University of Bristol, Interface Analysis Centre, H.H. Wills Physics Laboratory, Tyndall Avenue, Bristol BS8 1TL, United Kingdom.
University of Bristol, Interface Analysis Centre, H.H. Wills Physics Laboratory, Tyndall Avenue, Bristol BS8 1TL, United KingdombBiophotonics Research Unit, Gloucestershire Hospitals NHS Foundation Trust, Gloucester GL1 3NN, United Kingdom.
J Biomed Opt. 2016 Aug 1;21(8):87002. doi: 10.1117/1.JBO.21.8.087002.
Removal of intrinsic brain tumors is a delicate process, where a high degree of specificity is required to remove all of the tumor tissue without damaging healthy brain. The accuracy of this process can be greatly enhanced by intraoperative guidance. Optical biopsies using Raman spectroscopy are a minimally invasive and lower-cost alternative to current guidance methods. A miniature Raman probe for performing optical biopsies of human brain tissue is presented. The probe allows sampling inside a conventional stereotactic brain biopsy system: a needle of length 200 mm and inner diameter of 1.8 mm. By employing a miniature stand-off Raman design, the probe removes the need for any additional components to be inserted into the brain. Additionally, the probe achieves a very low internal silica background while maintaining good collection of Raman signal. To illustrate this, the probe is compared with a Raman probe that uses a pair of optical fibers for collection. The miniature stand-off Raman probe is shown to collect a comparable number of Raman scattered photons, but the Raman signal to background ratio is improved by a factor of five at Raman shifts below ∼500 cm(−1). The probe’s suitability for use on tissue is demonstrated by discriminating between different types of healthy porcine brain tissue.
切除颅内肿瘤是一个精细的过程，需要高度的特异性来切除所有肿瘤组织，而不损伤健康的大脑。术中指导可以极大地提高这个过程的准确性。使用拉曼光谱的光学活检是一种微创且成本更低的替代当前指导方法的方法。本文提出了一种用于对人脑组织进行光学活检的微型拉曼探头。该探头允许在传统的立体定向脑活检系统内进行采样：长度为 200mm 且内径为 1.8mm 的针。通过采用微型隔离拉曼设计，该探头无需插入任何额外的组件到大脑中。此外，该探头在保持良好拉曼信号采集的同时，实现了非常低的内部二氧化硅背景。为了说明这一点，将该探头与使用一对光纤进行收集的拉曼探头进行了比较。结果表明，微型隔离拉曼探头收集的拉曼散射光子数量相当，但在拉曼位移低于 ∼500 cm(−1)时，拉曼信号与背景的比值提高了五倍。该探头通过区分不同类型的健康猪脑组织，证明了其在组织上的适用性。
