Salihu M, Ajayi B O, Adedara I A, de Souza D, Rocha J B T, Farombi E O
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Departamento de Bioquímica e Biologia Molecular, CCNE, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Andrologia. 2017 Jun;49(5). doi: 10.1111/and.12658. Epub 2016 Aug 22.
This study evaluated the protective effects of 6-gingerol-rich fraction (6-GRF) from Zingiber officinale on carbendazim (CBZ)-induced reproductive toxicity in rats. Adult male rats were treated with either CBZ (50 mg/kg) alone or in combination with 6-GRF (50, 100 and 200 mg/kg) for 14 consecutive days. Gas chromatography-mass spectrometry (GCMS) analysis revealed that 6-GRF consists of ten bioactive chemical components with 6-gingerol being the most abundant (30.76%). Administration of 6-GRF significantly (p < .05) prevented CBZ-mediated increase in absolute and relative testes weights as well as restored the sperm quantity and quality in the treated rats to near control. In testes and epididymis, 6-GRF significantly abolished CBZ-mediated increase in oxidative damage as well as augmented antioxidant enzymes activities and glutathione level in the treated rats. Moreover, CBZ administration alone significantly decreased plasma levels of testosterone, thyrotropin, triiodothyronine and tetraiodothyronine, whereas follicle-stimulating hormone was significantly elevated without affecting luteinising hormone and prolactin levels when compared with the control. Conversely, 6-GRF ameliorated the disruption in the hormonal levels and restored their levels to near normalcy in CBZ-treated rats. Collectively, 6-GRF inhibited the adverse effects of CBZ on the antioxidant defence systems, hormonal balance and histology of the testes and epididymis in rats.
本研究评估了姜科植物姜黄中富含6-姜辣素的组分（6-GRF）对多菌灵（CBZ）诱导的大鼠生殖毒性的保护作用。成年雄性大鼠连续14天单独给予CBZ（50 mg/kg）或与6-GRF（50、100和200 mg/kg）联合给药。气相色谱-质谱联用（GCMS）分析表明，6-GRF由十种生物活性化学成分组成，其中6-姜辣素含量最高（30.76%）。给予6-GRF可显著（p < 0.05）防止CBZ介导的大鼠绝对和相对睾丸重量增加，并使治疗大鼠的精子数量和质量恢复至接近对照水平。在睾丸和附睾中，6-GRF可显著消除CBZ介导的氧化损伤增加，并增强治疗大鼠的抗氧化酶活性和谷胱甘肽水平。此外，与对照组相比，单独给予CBZ可显著降低血浆睾酮、促甲状腺激素、三碘甲状腺原氨酸和甲状腺素水平，而促卵泡激素显著升高，且不影响黄体生成素和催乳素水平。相反，6-GRF可改善CBZ治疗大鼠的激素水平紊乱，并使其水平恢复至接近正常。总体而言，6-GRF可抑制CBZ对大鼠抗氧化防御系统、激素平衡以及睾丸和附睾组织学的不良影响。
