Emmerich P, Jauch A, Hofmann M C, Cremer T, Walt H
Institute of Human Genetics and Anthropology, University of Heidelberg, Federal Republic of Germany.
Lab Invest. 1989 Aug;61(2):235-42.
A protocol was developed that allows determination of chromosome aberrations in interphase nuclei from paraffin embedded human tissues. As a model system tissue sections from xenografts derived from three testicular germ cell tumors (two teratocarcinoma and one embryonal carcinoma), as well as sections from normal intestine and testicular tissues, were hybridized with a biotin-labeled DNA probe specific for 1q12. For comparison, isolated nuclei in suspension and metaphase spreads from in vitro cell lines derived from the respective xenografts were assayed. Our results show that interphase tumor cells with aberrant numbers of chromosome 1 can be reliably detected both in vitro and in vivo. Subclones with two and three labeled chromosomes, respectively, could be defined and quantitatively evaluated. Present and future possibilities to pinpoint specific chromosome aberrations directly in cells present in body fluids or in tumor tissues are discussed.
我们开发了一种方案，可用于确定石蜡包埋的人体组织间期核中的染色体畸变。作为模型系统，将源自三种睾丸生殖细胞肿瘤（两种畸胎癌和一种胚胎癌）的异种移植组织切片，以及正常肠组织和睾丸组织的切片，与针对1q12的生物素标记DNA探针进行杂交。作为对照，对源自相应异种移植的体外细胞系的悬浮分离核和中期染色体铺展进行了检测。我们的结果表明，在体外和体内均可可靠地检测到1号染色体数目异常的间期肿瘤细胞。分别具有两条和三条标记染色体的亚克隆可以被定义并进行定量评估。本文还讨论了直接在体液或肿瘤组织中的细胞中精确检测特定染色体畸变的现有及未来可能性。
