Walt H, Emmerich P, Cremer T, Hofmann M C, Bannwart F
Institute of Pathology, University of Zürich, University Hospital, Switzerland.
Lab Invest. 1989 Nov;61(5):527-31.
The so-called atypical germ cells or cells of carcinoma in situ morphologically resemble neoplastic cells in seminoma. Since seminomas show numerical aberrations of chromosome 1 we have used a DNA probe specific for chromosome region 1q12 to determine whether such aberrations can be detected in atypical germ cell nuclei in paraffin-embedded seminiferous tubules as well. One-third of intratubular nuclei, containing atypical germ cells, consistently showed three hybridization signals in contrast to two signals regularly observed in normal intestine and in spermatogonia. We thus show that cytogenetic studies of precancerous cells can be performed directly on the tissue where these cells originate.
所谓的非典型生殖细胞或原位癌细胞在形态上类似于精原细胞瘤中的肿瘤细胞。由于精原细胞瘤显示出1号染色体的数目畸变，我们使用了一种对1q12染色体区域特异的DNA探针，以确定在石蜡包埋的生精小管中的非典型生殖细胞核中是否也能检测到这种畸变。与正常肠组织和精原细胞中通常观察到的两个信号相比，三分之一含有非典型生殖细胞的小管内细胞核始终显示出三个杂交信号。因此，我们表明可以直接在这些癌前细胞起源的组织上进行细胞遗传学研究。
