Colciago A, Melfi S, Giannotti G, Bonalume V, Ballabio M, Caffino L, Fumagalli F, Magnaghi V
Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano , Via G. Balzaretti 9, Milan 20133, Italy.
Cell Death Discov. 2015 Sep 7;1:15021. doi: 10.1038/cddiscovery.2015.21. eCollection 2015.
Previous evidence showed mutations of the neurofibromin type 2 gene (Nf2), encoding the tumor suppressor protein merlin, in sporadic and vestibular schwannomas affecting Schwann cells (SCs). Accordingly, efforts have been addressed to identify possible factors, even environmental, that may regulate neurofibromas growth. In this context, we investigated the exposure of SC to an electromagnetic field (EMF), which is an environmental issue modulating biological processes. Here, we show that SC exposed to 50 Hz EMFs changes their morphology, proliferation, migration and myelinating capability. In these cells, merlin is downregulated, leading to activation of two intracellular signaling pathways, ERK/AKT and Hippo. Interestingly, SC changes their phenotype toward a proliferative/migrating state, which in principle may be pathologically relevant for schwannoma development.
先前的证据表明，在散发性和前庭神经鞘瘤中，影响施万细胞（SCs）的神经纤维瘤病2型基因（Nf2）发生突变，该基因编码肿瘤抑制蛋白默林。因此，人们致力于确定可能调节神经纤维瘤生长的因素，甚至是环境因素。在此背景下，我们研究了施万细胞暴露于电磁场（EMF）的情况，电磁场是一个调节生物过程的环境问题。在这里，我们表明，暴露于50Hz电磁场的施万细胞会改变其形态、增殖、迁移和髓鞘形成能力。在这些细胞中，默林表达下调，导致细胞外调节蛋白激酶/蛋白激酶B（ERK/AKT）和河马（Hippo）两条细胞内信号通路激活。有趣的是，施万细胞将其表型转变为增殖/迁移状态，这原则上可能与神经鞘瘤的发生在病理上相关。
Zotero 插件