College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, 61 Daizong Road, Tai'an City 271000, Shandong Province, China.
Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases of Taishan Medical College, Tai'an City 271000, Shandong Province, China.
Sci Rep. 2016 Aug 24;6:32183. doi: 10.1038/srep32183.
Duck plague caused by duck plague virus (DPV) is an acute and contagious disease. To better understand the pathogenic mechanism of duck plague virus in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DPV could replicate quickly in many tissues, leading to pathological lesions, especially on the spleen. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the brain, and the antiviral innate immune response was established, but not sufficient to restrict viral replication. In contrast, although the expression of many major pattern recognition receptors (PRRs) increased in the spleen, the expression of most cytokines was declined. Our study indicates that DPV is a pantropic virus that can replicate rapidly in tissues, causing serious pathological lesions but the immune responses are different in the spleen and brain. To our knowledge, this is the first report to systematically explore the expression profiles of the immune genes in the DPV-infected ducks. Our data provide a foundation for further study of the pathogenicity of duck plague.
由鸭瘟病毒（DPV）引起的鸭瘟是一种急性、接触性传染病。为了更好地了解鸭瘟病毒在雏鸭中的致病机制，进行了感染实验。结果表明，感染雏鸭出现了典型症状。DPV 能在多种组织中快速复制，导致病理损伤，特别是脾脏。实时定量 PCR 表明，许多固有免疫相关基因在脑中的表达大多上调，建立了抗病毒固有免疫反应，但不足以限制病毒复制。相比之下，虽然脾脏中许多主要模式识别受体（PRRs）的表达增加，但大多数细胞因子的表达下降。我们的研究表明，DPV 是一种泛嗜性病毒，能在组织中快速复制，导致严重的病理损伤，但在脾脏和脑中的免疫反应不同。据我们所知，这是首次系统研究 DPV 感染鸭的免疫基因表达谱。我们的数据为进一步研究鸭瘟的致病性提供了基础。
