Aramvash Asieh, Gholami-Banadkuki Narges, Seyedkarimi Mansooreh-Sadat
Dept. of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran.
Biotechnol Prog. 2016 Nov;32(6):1480-1486. doi: 10.1002/btpr.2346. Epub 2016 Oct 21.
There are many published studies presenting ethanol and acetone as PHAs-poor solvents, where these two solvents are shown to dissolve <2% (w/v) of PHAs at low temperatures. In this study, the suitability of ethanol and acetone for the recovery of PHB at different temperatures (from room temperature to near boiling point) in Cupriavidus necator was investigated. Experiments were performed using response surface methodology to examine the effects of different temperatures and heating incubation times on recovery percentage using the two solvents. The highest recovery percentage (92.3%) and product purity (up to 99%) were obtained with ethanol-assisted extraction at 76°C for 32 min of incubation time. Under these conditions the extracted PHB exhibited a molecular mass of 1.2 × 10 . The present strategy showed that at temperatures near its boiling point, ethanol, as a nonhalogenated solvent, represents a good alternative to halogenated solvents, like chloroform, when PHB recovery is concerned. DSC analysis showed good thermal properties for ethanol- and acetone-extracted biopolymers. GC and H NMR analysis confirmed the extracted biopolymer to be polyhydroxybutyrate of good purity. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1480-1486, 2016.
有许多已发表的研究表明乙醇和丙酮是聚羟基脂肪酸酯（PHA）的不良溶剂，在低温下这两种溶剂溶解的PHA<2%（w/v）。在本研究中，考察了乙醇和丙酮在不同温度（从室温到接近沸点）下从食酸铜绿假单胞菌中回收聚羟基丁酸酯（PHB）的适用性。采用响应面法进行实验，研究不同温度和加热孵育时间对使用这两种溶剂回收百分比的影响。在76°C下孵育32分钟，乙醇辅助提取获得了最高回收率（92.3%）和产品纯度（高达99%）。在此条件下，提取的PHB分子量为1.2×10 。本策略表明，在接近其沸点的温度下，乙醇作为一种非卤代溶剂，在涉及PHB回收时，是氯仿等卤代溶剂的良好替代品。差示扫描量热法（DSC）分析表明乙醇和丙酮提取的生物聚合物具有良好的热性能。气相色谱（GC）和核磁共振氢谱（ 1H NMR）分析证实提取的生物聚合物是纯度良好的聚羟基丁酸酯。©2016美国化学工程师学会生物技术进展，32:1480 - 148,2016。
