García Gabriela, Sosa-Hernández Juan Eduardo, Rodas-Zuluaga Laura Isabel, Castillo-Zacarías Carlos, Iqbal Hafiz, Parra-Saldívar Roberto
Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, Nuevo Leon, Mexico.
Polymers (Basel). 2020 Dec 30;13(1):131. doi: 10.3390/polym13010131.
Traditional plastics have undoubted utility and convenience for everyday life; but when they are derived from petroleum and are non-biodegradable, they contribute to two major crises today's world is facing: fossil resources depletion and environmental degradation. Polyhydroxyalkanoates are a promising alternative to replace them, being biodegradable and suitable for a wide variety of applications. This biopolymer accumulates as energy and carbon storage material in various microorganisms, including microalgae. This study investigated the influence of glucose, N, P, Fe, and salinity over the production of polyhydroxyalkanoate (PHA) by sp., a freshwater microalga strain not previously explored for this purpose. To assess the effect of the variables, a fractional Taguchi experimental design involving 16 experimental runs was planned and executed. Biopolymer was obtained in all the experiments in a wide range of concentrations (0.83-29.92%, / DW), and identified as polyhydroxybutyrate (PHB) by FTIR analysis. The statistical analysis of the response was carried out using Minitab 16, where phosphorus, glucose, and iron were identified as significant factors, together with the P-Fe and glucose-N interactions. The presence of other relevant macromolecules was also quantified. Doing this, this work contributes to the understanding of the critical factors that control PHA production and present sp. as a promising species to produce bio-resources in commercial systems.
传统塑料在日常生活中具有毋庸置疑的实用性和便利性；但当它们由石油衍生而来且不可生物降解时，就会导致当今世界面临的两大危机：化石资源枯竭和环境退化。聚羟基脂肪酸酯是替代它们的一种有前景的选择，因为其具有生物可降解性且适用于多种应用。这种生物聚合物作为能量和碳储存物质积累在包括微藻在内的各种微生物中。本研究调查了葡萄糖、氮、磷、铁和盐度对一种淡水微藻菌株（此前未针对此目的进行过探索）生产聚羟基脂肪酸酯（PHA）的影响。为了评估这些变量的影响，计划并执行了一个包含16次实验运行的分数阶田口实验设计。在所有实验中均获得了浓度范围广泛（0.83 - 29.92%，/干重）的生物聚合物，并通过傅里叶变换红外光谱（FTIR）分析鉴定为聚羟基丁酸酯（PHB）。使用Minitab 16对响应进行统计分析，结果表明磷、葡萄糖和铁是显著因素，同时磷 - 铁和葡萄糖 - 氮的相互作用也很显著。还对其他相关大分子的存在进行了定量分析。通过这样做，本研究有助于理解控制PHA生产的关键因素，并表明该微藻菌株是在商业系统中生产生物资源的一个有前景的物种。
