使用前向激光散射技术的快速抗菌药敏试验
Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.
作者信息
Hayden Randall T, Clinton Lani K, Hewitt Carolyn, Koyamatsu Terri, Sun Yilun, Jamison Ginger, Perkins Rosalie, Tang Li, Pounds Stanley, Bankowski Matthew J
机构信息
St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA
Pathology Residency Program, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.
出版信息
J Clin Microbiol. 2016 Nov;54(11):2701-2706. doi: 10.1128/JCM.01475-16. Epub 2016 Aug 24.
The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.
抗菌药物敏感性试验结果的延迟报告仍然是细菌感染治疗临床决策中的一个限制因素。本研究评估了利用前向激光散射（FLLS）测量细菌生长以早期确定抗菌药物敏感性的方法。使用两种商业抗菌检测系统Vitek2和MicroScan MIC板对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的各三株菌株（两株临床分离株和一株参考菌株）进行了一式三份的测试，以检验BacterioScan FLLS。BacterioScan FLLS与商业方法显示出高度的类别一致性。以每种商业系统作为参考标准进行成对比较，结果显示与MicroScan的一致性为88.9%（两个小误差），与Vitek的一致性为72.2%（五个小误差）。使用BacterioScan系统的FLLS作为一种快速准确测定抗菌药物敏感性的新方法显示出了前景。
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