Department of Translational Research, National Cancer Institute - CRO-IRCSS, Aviano, Italy.
Doctoral School in Pharmacological Sciences, University of Padua, Padua, Italy.
Nanomedicine (Lond). 2016 Sep;11(18):2431-41. doi: 10.2217/nnm-2016-0154. Epub 2016 Aug 25.
To demonstrate that exosomes (exo) could increase the therapeutic index of doxorubicin (DOX).
MATERIALS & METHODS: Exosomes were characterized by nanoparticle tracking analysis and western blot. Tissue toxicity was evaluated by histopathological analysis and drug efficacy by measuring tumor volume. DOX biodistribution was analyzed by MS.
Exosomal doxorubicin (exoDOX) avoids heart toxicity by partially limiting the crossing of DOX through the myocardial endothelial cells. For this reason, mice can be treated with higher concentration of exoDOX thus increasing the efficacy of DOX as demonstrated in breast and ovarian mouse tumors.
ExoDOX is safer and more effective than free DOX. Importantly, the first spontaneous transformed syngeneic model of high-grade serous ovarian cancer was utilized for providing a new therapeutic opportunity.
证明外泌体（exo）可以提高阿霉素（DOX）的治疗指数。
通过纳米颗粒跟踪分析和 Western blot 对 exosomes 进行了表征。通过组织病理学分析评估组织毒性，通过测量肿瘤体积评估药物疗效。通过 MS 分析 DOX 的生物分布。
外泌体阿霉素（exoDOX）通过部分限制 DOX 通过心肌内皮细胞的穿越，从而避免了心脏毒性。因此，小鼠可以用更高浓度的 exoDOX 进行治疗，从而提高 DOX 的疗效，这在乳腺癌和卵巢癌小鼠肿瘤中得到了证实。
exoDOX 比游离 DOX 更安全、更有效。重要的是，首次利用自发转化的同源卵巢癌高级别浆液性癌模型为提供了新的治疗机会。
