Goldfarb Míriam, Santana Mateus Ferreira, Salomão Tânia Maria Fernandes, Queiroz Marisa Vieira de, Barros Everaldo Gonçalves de
Laboratório de Biologia Molecular de Plantas, Instituto de Biotecnologia Aplicada (BIOAGRO), Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
Laboratório de Genética Molecular e de Microrganismo, Instituto de Biotecnologia Aplicada (BIOAGRO), Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
Genet Mol Biol. 2016 Jul-Sep;39(3):426-30. doi: 10.1590/1678-4685-GMB-2015-0241. Epub 2016 Jul 7.
Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation) silencing mechanism that may directly affect the evolution of this species.
在核盘菌的基因组库中鉴定出了分别来自Copia和Gypsy超家族的两个逆转座子，分别命名为Copia-LTR_SS和Gypsy-LTR_SS。这些转座元件（TEs）包含直接且保留的长末端重复序列（LTR）。在Copia-LTR_SS中鉴定出了与gag蛋白、整合酶、逆转录酶和RNA酶H的编码区域相关的结构域，而在Gypsy-LTR_SS中仅发现了gag、逆转录酶和RNA酶H的结构域。已鉴定的LTR-单拷贝序列的丰度表明核盘菌基因组中可能存在基因重组事件。此外，每个超家族的LTR元件序列比对表明存在重复诱导点突变（RIP）沉默机制，这可能直接影响该物种的进化。
