Hillel J, Plotzy Y, Haberfeld A, Lavi U, Cahaner A, Jeffreys A J
Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Anim Genet. 1989;20(2):145-55. doi: 10.1111/j.1365-2052.1989.tb00852.x.
Human minisatellite probes cross-hybridize to DNA of several species of poultry (chicken, duck, turkey and goose), and detect high levels of polymorphism. The resulting DNA fingerprints are individual specific, and allow the discrimination even between closely related birds. The pattern of poultry DNA fingerprints is different from that of humans and other animals, having a higher average proportion of large DNA fragments. Pedigree analysis revealed a low number of allelic pairs of variable DNA fragments, indicating that most of the alleles are unresolved in the DNA fingerprint or too small to be detected. The total number of detectable loci in broilers, using probe 33.6, was estimated as 62, of which 13 loci are on average scoreable and available for use. Poultry DNA fingerprints can be used for individual identification, linkage studies and as an aid in breeding programmes.
人类小卫星探针可与几种家禽(鸡、鸭、火鸡和鹅)的DNA发生交叉杂交,并检测到高度的多态性。由此产生的DNA指纹具有个体特异性,甚至能区分亲缘关系很近的鸟类。家禽DNA指纹的模式与人类和其他动物不同,其大DNA片段的平均比例更高。系谱分析显示可变DNA片段的等位基因对数量较少,这表明大多数等位基因在DNA指纹中未得到解析或太小而无法检测到。使用探针33.6估计肉鸡中可检测到的基因座总数为62个,其中平均有13个基因座可计分并可供使用。家禽DNA指纹可用于个体识别、连锁研究以及辅助育种计划。