将 DNA“指纹”应用于苹果（Malus x domestica）的亲子分析。
DNA "fingerprints" applied to paternity analysis in apples (Malus x domestica).
机构信息
Biology Department, Washington University, Box 1137, 63130, Saint Louis, MO, USA.
出版信息
Theor Appl Genet. 1990 Jun;79(6):763-8. doi: 10.1007/BF00224242.
Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.
微卫星 DNA 序列分析产生了所谓的 DNA“指纹”，已被证明对几种不同生物的亲子关系分析很有用。这里使用 M13“指纹”探针分析了来自一个有三个品种的果园的种子生长的 64 个苹果幼苗。可以确定 56 个幼苗的亲子关系，其中 2 个是自交的。多等位基因位点的存在促进了分析。用 TaqI 或 RsaI 限制酶消化 DNA 时，该位点确定的五个不同片段迁移到相似的位置。
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