State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, the Third Military Medical University, China ; Chongqing Key Laboratory for Proteomics of Diseases, Chongqing, China.
Burns Trauma. 2014 Jan 26;2(1):24-8. doi: 10.4103/2321-3868.126088. eCollection 2014.
The frequency of T cells that can respond to alloantigens is unusually high. It remains unclear how T cells would respond when stimulated by multiple major histocompatibility complex (MHC) disparate alloantigens in the same cultures. In this report, we examined potential interactions of T cell clones that were stimulated simultaneously by two sets of complete MHC disparate alloantigens using mixed lymphocyte reaction (MLR). In this assay, we observed that proliferation of B6 lymphocytes (H-2b) stimulated by both BALB/c (H-2d) and C3H (H-2k) allogeneic cells was not increased but rather reduced as compared to B6 cells stimulated with either BALB/c or C3H allogeneic cells. Interestingly, interleukin (IL)-10 expressions at both protein level and mRNA level was significantly increased in cultures stimulated with the two MHC alloantigens, while IL-2, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1 production did not show any differences. In addition, Foxp3 mRNA expression was comparable amongst all groups. In conclusion, we observed an inhibitory effect in T cell proliferation in response to multiple MHC mismatched alloantigens in MLR, and this effect might be associated with the upregulation of IL-10 expression.
能够对同种异体抗原产生反应的 T 细胞的频率异常高。目前尚不清楚当 T 细胞在同一培养物中受到多种主要组织相容性复合物 (MHC) 不同同种异体抗原的刺激时会如何反应。在本报告中，我们使用混合淋巴细胞反应 (MLR) 检查了同时受到两组完全 MHC 不同同种异体抗原刺激的 T 细胞克隆之间的潜在相互作用。在该测定中，我们观察到与 B6 细胞受到 BALB/c（H-2d）或 C3H（H-2k）同种异体细胞刺激相比，B6 淋巴细胞（H-2b）的增殖既没有增加反而减少。有趣的是，与用 BALB/c 或 C3H 同种异体细胞刺激的培养物相比，用两种 MHC 同种异体抗原刺激的培养物中白细胞介素 (IL)-10 的蛋白水平和 mRNA 水平均显著增加，而 IL-2、肿瘤坏死因子 (TNF)-α、转化生长因子 (TGF)-β1 的产生则没有显示出任何差异。此外，Foxp3 mRNA 表达在所有组之间均无差异。总之，我们在 MLR 中观察到对多种 MHC 错配同种异体抗原的 T 细胞增殖产生抑制作用，这种作用可能与 IL-10 表达的上调有关。
