玉米黑粉菌病毒P4编码毒素的纯化及分子特性

Purification and molecular properties of the toxin coded by Ustilago maydis virus P4.

作者信息

Ganesa C, Chang Y J, Flurkey W H, Randhawa Z I, Bozarth R F

机构信息

Department of Life Sciences, Indiana State University, Terre Haute 47809.

出版信息

Biochem Biophys Res Commun. 1989 Jul 31;162(2):651-7. doi: 10.1016/0006-291x(89)92360-7.

DOI:10.1016/0006-291x(89)92360-7

PMID:2757636

Abstract

The toxin from the P4 strain of Ustilago maydis was purified and characterized using a series of gel-filtration and ion-exchange columns. The apparent molecular weight of the purified toxin was estimated from gel electrophoresis to be 11.3 kd in the presence of 2-mercaptoethanol and 10.3 kd in the absence of 2-mercaptoethanol. Amino acid analysis indicated 12% basic amino acids, 14% acidic amino acids and 16% glycine. The toxin was also stable to filtration and repeated freezing at -20 degrees C and thawing.

摘要

对玉米黑粉菌P4菌株产生的毒素进行了纯化，并使用一系列凝胶过滤柱和离子交换柱对其特性进行了表征。通过凝胶电泳估计，纯化毒素在存在2-巯基乙醇的情况下表观分子量为11.3千道尔顿，在不存在2-巯基乙醇的情况下为10.3千道尔顿。氨基酸分析表明，碱性氨基酸占12%，酸性氨基酸占14%，甘氨酸占16%。该毒素对过滤以及在-20℃下反复冷冻和解冻也具有稳定性。