Taslidere E, Dogan Z, Elbe H, Vardi N, Cetin A, Turkoz Y
a Department of Histology and Embryology , Faculty of Medicine, Bezmialem Vakif University , Istanbul , Turkey.
b Department of Anatomy , Faculty of Medicine, Adiyaman University , Adiyaman , Turkey.
Biotech Histochem. 2016;91(2):116-21. doi: 10.3109/10520295.2015.1085093. Epub 2015 Nov 3.
Ciprofloxacin is a common, broad spectrum antibacterial agent; however, evidence is accumulating that ciprofloxacin may cause liver damage. Quercetin is a free radical scavenger and antioxidant. We investigated histological changes in hepatic tissue of rats caused by ciprofloxacin and the effects of quercetin on these changes using histochemical and biochemical methods. We divided 28 adult female Wistar albino rats into four equal groups: control, quercetin treated, ciprofloxacin treated, and ciprofloxacin + quercetin treated. At the end of the experiment, liver samples were processed for light microscopic examination and biochemical measurements. Sections were prepared and stained with hematoxylin and eosin, and a histopathologic damage score was calculated. The sections from the control group appeared normal. Hemorrhage, inflammatory cell infiltration and intracellular vacuolization were observed in the ciprofloxacin group. The histopathological findings were reduced in the group treated with quercetin. Significant differences were found between the control and ciprofloxacin groups, and between the ciprofloxacin and ciprofloxacin + quercetin groups. Quercetin administration reduced liver injury caused by ciprofloxacin in rats. We suggest that quercetin may be useful for preventing ciprofloxacin induced liver damage.
环丙沙星是一种常用的广谱抗菌剂；然而，越来越多的证据表明环丙沙星可能会导致肝损伤。槲皮素是一种自由基清除剂和抗氧化剂。我们使用组织化学和生物化学方法研究了环丙沙星引起的大鼠肝组织的组织学变化以及槲皮素对这些变化的影响。我们将28只成年雌性Wistar白化大鼠平均分为四组：对照组、槲皮素处理组、环丙沙星处理组和环丙沙星+槲皮素处理组。实验结束时，对肝脏样本进行处理以进行光学显微镜检查和生化测量。制备切片并用苏木精和伊红染色，计算组织病理学损伤评分。对照组的切片看起来正常。在环丙沙星组中观察到出血、炎性细胞浸润和细胞内空泡化。在槲皮素处理组中，组织病理学结果有所减轻。在对照组和环丙沙星组之间以及环丙沙星组和环丙沙星+槲皮素组之间发现了显著差异。给予槲皮素可减轻环丙沙星对大鼠造成的肝损伤。我们认为槲皮素可能有助于预防环丙沙星诱导的肝损伤。
