Mardas Nikos, Busetti Juliano, de Figueiredo José Antonio Poli, Mezzomo Luis André, Scarparo Roberta Kochenborger, Donos Nikolaos
Centre for Adult Oral Health, Institute of Dentistry, Bart's & The London School of Medicine & Dentistry, Queen Mary University, London, UK.
Dental School, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Clin Oral Implants Res. 2017 Mar;28(3):362-371. doi: 10.1111/clr.12810. Epub 2016 Feb 26.
To evaluate new bone formation in calvarial critical size defects (CSD) under dense polytetrafluoroethylene (d-PTFE), microporous membranes for guided bone regeneration (GBR) in healthy, osteoporotic and osteoporotic treated with zoledronic acid (ZA) rats.
Forty-eight, female, 6-month old Wistar rats were included in the study. Osteoporosis was induced by ovariectomy (OVX) and calcium-deficient diet in 32 rats. Sixteen OVX rats were treated with a single dose of Zolendronic Acid (ZA) (OZ), while 16 OVX rats received no treatment (O). The remaining 16 rats were sham-operated and used as healthy controls (C). At 6 weeks following osteoporosis induction, two 5 mm CSD were created in the parietal bones and one of them was treated with a double d-PTFE membrane. The healing periods were 30 and 60 days. New bone formation (NB) was assessed by qualitative and quantitative histological analysis.
After 30 days of healing, NB (mean% (95% CI)) was 78.9% (21), 93.1% (9.3) and 84.2% (26.9) in the membrane treated defects and 18.8% (24.1), 27.1% (7.9) and 31% (38.8) in the untreated defects of group O, OZ and C, respectively. After 60 days of healing, NB was 78.3% (14.4), 95.8% (9) and 90.1% (26.1) in the membrane treated defects and 10.8% (17.4), 51.6% (39.4) and 15.7% (12.1) in the untreated defects of group O, OZ and C, respectively. Hierarchical analysis of variance showed that treatment with ZA (P = 0.001) and the use of membrane (P = 0.000) significantly increased new bone formation while presence of osteoporosis may have reduced new bone formation (P = 0.028).
d-PTFE membranes for GBR promote bone healing in osteoporotic and healthy rats. Treatment with ZA may improve new bone formation in osteoporotic rats.
评估在健康、骨质疏松以及接受唑来膦酸（ZA）治疗的骨质疏松大鼠颅骨临界尺寸缺损（CSD）处，致密聚四氟乙烯（d-PTFE）微孔膜引导骨再生（GBR）情况下的新骨形成情况。
48只6月龄雌性Wistar大鼠纳入本研究。32只大鼠通过卵巢切除术（OVX）和低钙饮食诱导骨质疏松。16只OVX大鼠接受单剂量唑来膦酸（ZA）治疗（OZ组），16只OVX大鼠未接受治疗（O组）。其余16只大鼠进行假手术并作为健康对照（C组）。在诱导骨质疏松6周后，在顶骨制造两个5mm的CSD，其中一个用双层d-PTFE膜治疗。愈合期为30天和60天。通过定性和定量组织学分析评估新骨形成（NB）情况。
愈合30天后，在膜治疗的缺损处，O组、OZ组和C组未治疗缺损处的NB（平均百分比（95%CI））分别为78.9%（21）、93.1%（9.3）和84.2%（26.9），以及18.8%（24.1）、27.1%（7.9）和31%（38.8）。愈合60天后，在膜治疗的缺损处，O组、OZ组和C组未治疗缺损处的NB分别为78.3%（14.4）、95.8%（9）和90.1%（26.1），以及10.8%（17.4）、51.6%（39.4）和15.7%（12.1）。方差分层分析显示，ZA治疗（P = 0.001）和使用膜（P = 0.000）显著增加新骨形成，而骨质疏松的存在可能减少新骨形成（P = 0.028）。
用于GBR的d-PTFE膜可促进骨质疏松和健康大鼠的骨愈合。ZA治疗可能改善骨质疏松大鼠的新骨形成。
