Ensor D M, Morley J S, Redfern R M, Miles J B
Department of Environmental and Evolutionary Biology, University of Liverpool, U.K.
Brain Res. 1989 Jun 19;490(1):196-9. doi: 10.1016/0006-8993(89)90452-6.
The output of dopamine from cultures of rat adrenal medullary strips was increased by 71-120% when Lys-Lys-Gly-Glu (MPF), the C-terminal tetrapeptide sequence of human beta-endorphin, was added to the culture medium at 100 micrograms/ml concentration. Human beta-endorphin caused a 44% increase, but an N-terminal fragment of its molecule and somatotrophin caused no increase. Results with analogs of MPF show that small structural change of the C-terminal Glu residue causes complete loss of activity.
当以100微克/毫升的浓度将人β-内啡肽的C末端四肽序列Lys-Lys-Gly-Glu（MPF）添加到培养基中时，大鼠肾上腺髓质条带培养物中多巴胺的产量增加了71-120%。人β-内啡肽使产量增加了44%，但其分子的N末端片段和生长激素则没有使产量增加。MPF类似物的结果表明，C末端Glu残基的微小结构变化会导致活性完全丧失。
