Austin J, Kimble J
Department of Genetics, University of Wisconsin, Madison 53706.
Cell. 1989 Aug 11;58(3):565-71. doi: 10.1016/0092-8674(89)90437-6.
The glp-1 and lin-12 genes mediate several cell interactions during C. elegans development. We have identified the glp-1 gene in a region about 20 kb from lin-12. In collaboration with Yochem and Greenwald (1989; see accompanying paper), we show that a sequence identified by its similarity to lin-12 is in fact glp-1. We find a single 4.4 kb glp-1 transcript and a distinct 4.6 kb lin-12 transcript. Expression of the glp-1 transcript during development differs from that of lin-12. As expected from genetic analyses, glp-1 RNA is primarily in the germline while lin-12 RNA is primarily in the soma. Unexpectedly, we find that glp-1 RNA is also expressed in larval somatic tissues and that lin-12 RNA is abundant in early embryos. We suggest that glp-1 and lin-12 may play broader roles in development than previously thought.
在秀丽隐杆线虫的发育过程中，glp-1基因和lin-12基因介导了多种细胞间相互作用。我们在距离lin-12约20 kb的区域内鉴定出了glp-1基因。与约切姆和格林瓦尔德合作（1989年；见随附论文），我们发现一个因其与lin-12的相似性而被鉴定出的序列实际上就是glp-1。我们发现了一个4.4 kb的单一glp-1转录本和一个不同的4.6 kb的lin-12转录本。glp-1转录本在发育过程中的表达与lin-12不同。正如遗传分析所预期的那样，glp-1 RNA主要存在于生殖系中，而lin-12 RNA主要存在于体细胞中。出乎意料的是，我们发现glp-1 RNA也在幼虫的体细胞组织中表达，并且lin-12 RNA在早期胚胎中大量存在。我们认为，glp-1和lin-12在发育过程中可能发挥着比之前认为的更广泛的作用。
