Yochem J, Greenwald I
Department of Biology, Princeton University, New Jersey 08544-1003.
Cell. 1989 Aug 11;58(3):553-63. doi: 10.1016/0092-8674(89)90436-4.
Genomic DNA closely related in sequence to lin-12, a gene that specifies certain cell fates during C. elegans development, was isolated from a C. elegans library by low stringency hybridization. DNA sequencing of genomic and cDNA clones predicts the new sequence to encode an integral membrane protein that shares three repeated amino acid sequence motifs with the lin-12 product and the Drosophila Notch product: an epidermal growth factor-like motif, the "lin-12/Notch Repeat," and a motif present in two yeast gene products that have cell cycle dependent functions. Austin and Kimble (see accompanying paper) present evidence that this sequence corresponds to glp-1, a gene implicated in cell-cell interactions distinct from those involving lin-12. Possible implications of the predicted structure of the glp-1 product with respect to these cell-cell interactions are discussed.
通过低严谨度杂交从秀丽隐杆线虫文库中分离出了与lin-12序列密切相关的基因组DNA，lin-12是一个在秀丽隐杆线虫发育过程中决定某些细胞命运的基因。对基因组和cDNA克隆进行DNA测序预测，该新序列编码一种整合膜蛋白，它与lin-12产物和果蝇Notch产物共有三个重复的氨基酸序列基序：一个表皮生长因子样基序、“lin-12/Notch重复序列”，以及存在于两种具有细胞周期依赖性功能的酵母基因产物中的一个基序。奥斯汀和金布尔（见随附论文）提供的证据表明，该序列对应于glp-1，一个与不同于涉及lin-12的细胞间相互作用有关的基因。讨论了glp-1产物的预测结构对这些细胞间相互作用的可能影响。
