Roointan A, Sharifi-Rad M, Badrzadeh F, Sharifi-Rad J
Shiraz university of Medical sciences Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies Shiraz Iran.
Zabol University of Medical Sciences Zabol Iran.
Cell Mol Biol (Noisy-le-grand). 2016 Aug 29;62(9):51-6.
Lung cancer is the most common cancer among men. Since the main reason of cancer cells immortality is telomerase activity, targeting of such enzyme can be a promising approach in cancer therapy. Curcumin is a safe and efficient anticancer agent in this context, but its applications in cancer therapy are limited because of its hydrophobic structure and low solubility in water. Today, using nanocarriers for delivery of such anticancer agents is a well performed method. Here, we developed and compared the efficiency of two nanocarriers (PLGA-PEG and NIPAAm-MAA) in delivery of curcumin and also in levels of hTERT silencing in lung cancer cell line (calu-6). Scanning electron microscopy, MTT assays and real-time PCR were used for imaging, cytotoxicity testing and measuring the expression levels of hTERT after treatment of cells with different concentrations of free curcumin and curcumin loaded nanocarriers. The MTT results demonstrated that the IC50 values of curcumin loaded nanocarriers were in lower concentrations than free curcumin. The hTERT expression levels were decreased by curcumin loaded PLGA-PEG more than curcumin loaded NIPAAm-MAA and free curcumin. Our results showed that the curcumin loaded PLGA-PEG can be a useful nano based carrier for delivery of anti-cancer agents such as curcumin to fight lung cancer.
肺癌是男性中最常见的癌症。由于癌细胞永生的主要原因是端粒酶活性，靶向这种酶可能是癌症治疗中一种有前景的方法。姜黄素在这方面是一种安全有效的抗癌剂，但其在癌症治疗中的应用受到限制，因为其疏水结构和在水中的低溶解度。如今，使用纳米载体递送此类抗癌剂是一种行之有效的方法。在此，我们开发并比较了两种纳米载体（聚乳酸-羟基乙酸共聚物-聚乙二醇（PLGA-PEG）和N-异丙基丙烯酰胺-甲基丙烯酸（NIPAAm-MAA））在递送姜黄素方面的效率，以及它们在肺癌细胞系（calu-6）中对人端粒酶逆转录酶（hTERT）沉默水平的影响。使用扫描电子显微镜、MTT法和实时聚合酶链反应（PCR）对用不同浓度的游离姜黄素和负载姜黄素的纳米载体处理后的细胞进行成像、细胞毒性测试和测量hTERT的表达水平。MTT结果表明，负载姜黄素的纳米载体的半数抑制浓度（IC50）值低于游离姜黄素。负载姜黄素的PLGA-PEG比负载姜黄素的NIPAAm-MAA和游离姜黄素更能降低hTERT的表达水平。我们的结果表明，负载姜黄素的PLGA-PEG可以作为一种有用的纳米载体，用于递送姜黄素等抗癌剂来对抗肺癌。
