Badrzadeh Fariba, Akbarzadeh Abolfazl, Zarghami Nosratollah, Yamchi Mohammad Rahmati, Zeighamian Vahide, Tabatabae Fateme Sadate, Taheri Morteza, Kafil Hossein Samadi
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran E-mail :
Asian Pac J Cancer Prev. 2014;15(20):8931-6. doi: 10.7314/apjcp.2014.15.20.8931.
Herbal compounds such as curcumin which decrease telomerase and gene expression have been considered as beneficial tools for lung cancer treatment. In this article, we compared the effects of pure curcumin and curcumin-loaded NIPAAm-MAA nanoparticles on telomerase and PinX1 gene expression in a lung cancer cell line.
A tetrazolium-based assay was used for determination of cytotoxic effects of curcumin on the Calu-6 lung cancer cell line and telomerase and pinX1 gene expression was measured with real-time PCR.
MTT assay showed that Curcumin-loaded NIPAAm-MAA inhibited the growth of the Calu-6 lung cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of curcumin-loaded NIPAAm-MAA increased while expression of the PinX1 gene became elevated.
The results showed that curcumin- loaded- NIPAAm-MAA exerted cytotoxic effects on the Calu-6 cell line through down-regulation of telomerase and stimulation of pinX1 gene expression. NIPPAm-MAA could be good carrier for such kinds of hydrophobic agent.
姜黄素等能降低端粒酶和基因表达的草药化合物被视为肺癌治疗的有益工具。在本文中，我们比较了纯姜黄素和载姜黄素的NIPAAm-MAA纳米颗粒对肺癌细胞系中端粒酶和PinX1基因表达的影响。
采用基于四唑盐的检测方法测定姜黄素对Calu-6肺癌细胞系的细胞毒性作用，并用实时PCR检测端粒酶和pinX1基因表达。
MTT检测表明，载姜黄素的NIPAAm-MAA以时间和剂量依赖的方式抑制Calu-6肺癌细胞系的生长。我们的q-PCR结果显示，随着载姜黄素的NIPAAm-MAA浓度增加，端粒酶基因表达有效降低，而PinX1基因表达升高。
结果表明，载姜黄素的NIPAAm-MAA通过下调端粒酶和刺激pinX1基因表达对Calu-6细胞系发挥细胞毒性作用。NIPPAm-MAA可能是此类疏水药物的良好载体。
