Bagheri Raana, Sanaat Zohreh, Zarghami Nosratollah
Department of Molecular Genetics, Islamic Azad University, Tabriz, Iran.
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Res (Stuttg). 2018 Jun;68(6):335-343. doi: 10.1055/s-0043-121338. Epub 2018 Jan 2.
Telomerase is known as a global therapeutic target in cancer cells due to its main role in tumorigenesis. Nowadays, it is proposed new treatment methods based on molecular target therapy by bioactive substances such as curcumin and chrysin with fewer side effects than other chemical drugs. But due to their low aqueous solubility and high clearance in the bloodstream it can be used of nanoparticles to increase their half-life and biocompatibility of them. Therefore, the goal of this study was to evaluate the effect of Chrysin-Curcumin on the expression of telomerase gene in SW480 colorectal cancer cell line.
PLGA-PEG nanoparticles synthesized and were confirmed using by the scanning electron microscope (SEM) and FTIR Spectroscopy. After treatment of SW480 cells by curcumin and chrysin loaded nanoparticles, their toxicity to cancer cells, was evaluated by MTT. Then, the inhibition of hTERT gene expression was measured using qRT-PCR method.
The results of MTT test showed nanocapsulated curcumin and chrysin compared with free forms of these compounds have high synergistic effect on sw480 cells. Also, real time-PCR showed significant decrease in hTERT gene expression in SW480 cells that treated with nano-curcumin and nano-chrysin compare to untreated cells.
Nano-encapsulation of curcumin and chrysin enhanced delivery of these compounds to SW480 colorectal cancer cells and therefore it can be conclude that PLGA-PEG nanoparticles promote anticancer effects of curcumin-chrysin by increasing bioavailability and the solubility of these drugs.
端粒酶因其在肿瘤发生中的主要作用而被认为是癌细胞的一个重要治疗靶点。如今，人们提出了基于分子靶向治疗的新方法，使用姜黄素和白杨素等生物活性物质，其副作用比其他化学药物更少。但由于它们在水中的溶解度低且在血液中清除率高，因此可以使用纳米颗粒来延长它们的半衰期并提高其生物相容性。因此，本研究的目的是评估白杨素 - 姜黄素对SW480结肠癌细胞系中端粒酶基因表达的影响。
合成了PLGA - PEG纳米颗粒，并通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）进行了确认。用负载姜黄素和白杨素的纳米颗粒处理SW480细胞后，通过MTT法评估它们对癌细胞的毒性。然后，使用qRT - PCR方法测量hTERT基因表达的抑制情况。
MTT试验结果表明，与这些化合物的游离形式相比，纳米包裹的姜黄素和白杨素对SW480细胞具有高协同效应。此外，实时PCR显示，与未处理的细胞相比，用纳米姜黄素和纳米白杨素处理的SW480细胞中hTERT基因表达显著降低。
姜黄素和白杨素的纳米包封增强了这些化合物向SW480结肠癌细胞的递送，因此可以得出结论，PLGA - PEG纳米颗粒通过提高这些药物的生物利用度和溶解度来促进姜黄素 - 白杨素的抗癌作用。
