Bush Joshua R, Liang Haixiang, Dickinson Molly, Botchwey Edward A
Department of Orthopaedic Surgery, University of Virginia, PO Box 800374, Charlottesville, VA, 22908, USA.
Department of Biomedical Engineering, University of Virginia, PO Box 800359, Charlottesville, VA, 22908, USA.
Polym Adv Technol. 2016 Aug;27(8):1050-1055. doi: 10.1002/pat.3767. Epub 2016 Feb 2.
The hemicellulose xylan, which has immunomodulatory effects, has been combined with chitosan to form a composite hydrogel to improve the healing of bone fractures. This thermally responsive and injectable hydrogel, which is liquid at room temperature and gels at physiological temperature, improves the response of animal host tissue compared with similar pure chitosan hydrogels in tissue engineering models. The composite hydrogel was placed in a subcutaneous model where the composite hydrogel is replaced by host tissue within 1 week, much earlier than chitosan hydrogels. A tibia fracture model in mice showed that the composite encourages major remodeling of the fracture callus in less than 4 weeks. A non-union fracture model in rat femurs was used to demonstrate that the composite hydrogel allows bone regeneration and healing of defects that with no treatment are unhealed after 6 weeks. These results suggest that the xylan/chitosan composite hydrogel is a suitable bone graft substitute able to aid in the repair of large bone defects.
具有免疫调节作用的半纤维素木聚糖已与壳聚糖结合形成复合水凝胶，以促进骨折愈合。这种热响应性可注射水凝胶在室温下为液体，在生理温度下凝胶化，与组织工程模型中类似的纯壳聚糖水凝胶相比，改善了动物宿主组织的反应。将复合水凝胶置于皮下模型中，1周内复合水凝胶被宿主组织替代，比壳聚糖水凝胶早得多。小鼠胫骨骨折模型表明，该复合材料在不到4周的时间内促进了骨折痂的主要重塑。大鼠股骨骨不连骨折模型用于证明复合水凝胶能够实现骨再生并治愈缺损，未经治疗的缺损在6周后仍未愈合。这些结果表明，木聚糖/壳聚糖复合水凝胶是一种合适的骨移植替代物，能够帮助修复大的骨缺损。
