Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, 6627, Av Antônio Carlos, Engineering School, Block 2, 31.270-901 Belo Horizonte, MG, Brazil.
Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, 6627, Av Antônio Carlos, Engineering School, Block 2, 31.270-901 Belo Horizonte, MG, Brazil.
Int J Biol Macromol. 2019 Jul 1;132:811-821. doi: 10.1016/j.ijbiomac.2019.03.237. Epub 2019 Apr 1.
The present work describes in vitro and in vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles. Assays in SBF (simulated body fluid) solution showed that loss of hydrogel mass in vitro was decreased by 4.3% when bioactive glass nanoparticles (nBG) were incorporated, and confirmed the bioactivity of nBG containing hydrogels. In vitro assays demonstrated the cytocompatibility of the hydrogels with encapsulated rat bone marrow mesenchymal stem cells (BMSC). Crystal violet assays showed a 27% increase in cell viability when these cells were seeded in hydrogels containing nBG. In vivo biocompatibility was examined by injecting hydrogels into the dorsum of Swiss rats. The results indicated that the prepared hydrogels were nontoxic upon subcutaneous injection, and could be candidates for a safe in situ gel-forming system. Injection of the hydrogels into a rat tibial defect allowed preliminary evaluation of the hydrogels' regenerative potential. Micro Computed Tomography analysis suggested that more new tissue was formed in the defects treated with the hydrogels. Taken together, our data suggest that the developed injectable composite hydrogels possess properties which make them suitable candidates for use as temporary injectable matrices for bone regeneration.
本工作描述了基于聚合物/生物活性玻璃纳米粒子的温敏复合水凝胶的体外和体内行为。在 SBF（模拟体液）溶液中的实验表明，当掺入生物活性玻璃纳米粒子（nBG）时，水凝胶的体外质量损失减少了 4.3%，并证实了含有 nBG 的水凝胶的生物活性。体外实验证明了水凝胶对包封的大鼠骨髓间充质干细胞（BMSC）的细胞相容性。结晶紫分析表明，当这些细胞接种在含有 nBG 的水凝胶中时，细胞活力增加了 27%。通过将水凝胶注入瑞士大鼠的背部来检查体内生物相容性。结果表明，皮下注射制备的水凝胶无毒性，可作为安全的原位凝胶形成系统的候选物。将水凝胶注入大鼠胫骨缺损中允许对水凝胶的再生潜力进行初步评估。微计算机断层扫描分析表明，在接受水凝胶治疗的缺陷中形成了更多的新组织。总之，我们的数据表明，所开发的可注射复合水凝胶具有适合用作骨再生临时可注射基质的特性。
