Sirota T V, Zakharchenko M V, Kondrashova M N
Biomed Khim. 2014 Jan-Feb;60(1):63-71. doi: 10.18097/pbmc20146001063.
Several parameters of the cytoplasmic enzymatic antioxidant system of the liver and brain of the rat have been investigated under conditions of immobilization stress and of an antioxidant preparation in the diet of animals. These included superoxide dismutase (SOD) and glutathione reductase (GR) activities and nonspecific NADPH oxidation. Only changes in the activity of SOD both in the liver and brain were revealed. In the liver of animals that receive no preparation, a decrease in the activity of SOD after 30-min immobilization and its restoration after a 360-min immobilization were observed. In the brain, the activity of SOD decreased only in preconditioned animals after 30 and 360 min of exposure to stress. In addition, the activity of SOD in the brain of preconditioned animals, both stressed and unstressed, was lower than in the corresponding groups of control animals. It is probable that, under the conditions of immobilization stress, the level of reactive oxygen species (ROS) and as a consequence the activity of SOD decrease. The intake of an antioxidant preparation under these conditions seems to be not correct.
在动物饮食中添加抗氧化剂制剂的情况下，对大鼠肝脏和大脑细胞质酶抗氧化系统的几个参数进行了研究，研究条件包括固定应激。这些参数包括超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GR）活性以及非特异性NADPH氧化。仅发现肝脏和大脑中SOD活性发生了变化。在未接受制剂的动物肝脏中，观察到固定30分钟后SOD活性降低，固定360分钟后恢复。在大脑中，仅在预处理动物暴露于应激30分钟和360分钟后SOD活性降低。此外，预处理动物（无论是否应激）大脑中的SOD活性均低于相应对照组动物。在固定应激条件下，活性氧（ROS）水平可能会降低，从而导致SOD活性下降。在这些条件下摄入抗氧化剂制剂似乎并不合适。
